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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 9, pp 2383–2391 | Cite as

Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry

  • Ling-Na Zheng
  • Meng Wang
  • Lei-Chao Zhao
  • Bao-Yun Sun
  • Bing Wang
  • Han-Qing Chen
  • Yu-Liang Zhao
  • Zhi-Fang Chai
  • Wei-Yue FengEmail author
Research Paper
Part of the following topical collections:
  1. Spectrochemical Plasmas for Clinical and Biochemical Analysis

Abstract

Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C82(OH)22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C82(OH)22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C82(OH)22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.

Graphical Abstract

The quantitative results of Gd@C82(OH)22 in single cells determined by SC-ICP-MS and acid digestion method, respectively

Keyword

Cisplatin Gd@C82(OH)22 Single cell ICP-MS Quantitative analysis 

Notes

Acknowledgments

This work was supported by the National Basic Research Program (973 Program: 2011CB933403) and the National Natural Science Foundation of China (21175136, 11275214, and 11375211).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Ling-Na Zheng
    • 1
  • Meng Wang
    • 1
  • Lei-Chao Zhao
    • 1
    • 2
  • Bao-Yun Sun
    • 1
  • Bing Wang
    • 1
  • Han-Qing Chen
    • 1
  • Yu-Liang Zhao
    • 1
    • 3
  • Zhi-Fang Chai
    • 1
  • Wei-Yue Feng
    • 1
    Email author
  1. 1.CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy PhysicsChinese Academy of SciencesBeijingChina
  2. 2.School of Materials Science and EngineeringHebei University of TechnologyTianjinChina
  3. 3.CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of ChinaChinese Academy of SciencesBeijingChina

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