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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 6, pp 1745–1752 | Cite as

Molecularly imprinted microspheres synthesized by a simple, fast, and universal suspension polymerization for selective extraction of the topical anesthetic benzocaine in human serum and fish tissues

  • Hui Sun
  • Jia-Ping LaiEmail author
  • Fang Chen
  • De-Rong Zhu
Research Paper

Abstract

A simple, fast, and universal suspension polymerization method was used to synthesize the molecularly imprinted microspheres (MIMs) for the topical anesthetic benzocaine (BZC). The desired diameter (10–20 μm) and uniform morphology of the MIMs were obtained easily by changing one or more of the synthesis conditions, including type and amount of surfactant, stirring rate, and ratio of organic to water phase. The MIMs obtained were used as a molecular-imprinting solid-phase-extraction (MISPE) material for extraction of BZC in human serum and fish tissues. The MISPE results revealed that the BZC in these biosamples could be enriched effectively after the MISPE operation. The recoveries of BZC on MIMs cartridges were higher than 90 % (n = 3). Finally, an MISPE-HPLC method with UV detection was developed for highly selective extraction and fast detection of trace BZC in human serum and fish tissues. The developed method could also be used for the enrichment and detection of BZC in other complex biosamples.

Keywords

Molecularly imprinted microspheres Benzocaine Aqueous-suspension polymerization Solid-phase extraction Human serum Fish tissues 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (21107018, 21477026), Science and Technology Key Project of Ministry of Education (212129), Natural Science Foundation of Guangdong Province (S2012010008172).

Supplementary material

216_2014_8420_MOESM1_ESM.pdf (450 kb)
ESM 1 (PDF 317 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.College of Environmental Science & EngineeringGuangzhou UniversityGuangzhouChina
  2. 2.School of Chemistry & EnvironmentSouth China Normal UniversityGuangzhouChina
  3. 3.School of PharmacyGuangdong Medical CollegeDongwanChina

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