Analytical and Bioanalytical Chemistry

, Volume 407, Issue 2, pp 509–519 | Cite as

Molecularly imprinted polymers based on SBA-15 for selective solid-phase extraction of baicalein from plasma samples

  • Hongliang He
  • Xiaoli Gu
  • Liying Shi
  • Junli Hong
  • Hongjuan Zhang
  • Yankun Gao
  • Shuhu Du
  • Lina ChenEmail author
Research Paper


Highly selective molecularly imprinted mesoporous silica polymer (SBA-15@MIP) for baicalein (BAI) extraction was synthesized using a surface molecular imprinting technique on the SBA-15 supporter. Computational simulation was used to predict the optimal functional monomer for the rational design of SBA-15@MIP. Meanwhile, high adsorption capacity was obtained when a suitable yield of molecularly imprinted polymers (MIPs) layer was grafted onto the surface of SBA-15. Characterization and performance tests of the obtained polymer revealed that SBA-15@MIP possessed a highly ordered mesoporous structure, reached saturated adsorption within 60 min, and exhibited higher sorption capacity to the target molecule BAI compared with non-imprinted mesoporous silica polymer (SBA-15@NIP) and SBA-15. Finally, SBA-15@MIP was successfully applied to solid-phase extraction (SPE) coupled with high-performance liquid chromatography and ultraviolet detection (HPLC-UV) for the determination of trace BAI in plasma samples. Mean recoveries of BAI through the molecularly imprinted solid-phase extraction (MISPE) sorbent, non-imprinted solid-phase extraction (NISPE) sorbent, and SBA-15 solid-phase extraction (SBA-15-SPE) sorbent were 94.4, 22.7, and 10.7 %, respectively, and the relative standard deviations were 2.9, 2.6, and 3.6 %, respectively. These results reveal that SBA-15@MIP as a SPE sorbent has good applicability to selectively separate and enrich trace BAI from complex samples.

Graphical Abstract

Synthesis procedure of SBA-15@MIP


Surface molecular imprinting SBA-15 Baicalein Solid-phase extraction Computational simulation Plasma 



This work was financially supported by the National Natural Science Foundation of China (Nos. 30801558, 81173538, 21075066, 81373895).

Conflict of interest

The authors have declared no conflict of interest.

Supplementary material

216_2014_8285_MOESM1_ESM.pdf (838 kb)
ESM 1 (PDF 838 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Hongliang He
    • 1
  • Xiaoli Gu
    • 1
  • Liying Shi
    • 1
  • Junli Hong
    • 1
  • Hongjuan Zhang
    • 1
  • Yankun Gao
    • 1
  • Shuhu Du
    • 1
  • Lina Chen
    • 1
    Email author
  1. 1.School of PharmacyNanjing Medical UniversityNanjingChina

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