Analytical and Bioanalytical Chemistry

, Volume 407, Issue 2, pp 557–567 | Cite as

Determination of benzimidazole anthelmintics in milk and honey by monolithic fiber-based solid-phase microextraction combined with high-performance liquid chromatography–diode array detection

Research Paper
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Abstract

A porous poly(methacrylic acid-co-ethylene dimethacrylate) monolithic fiber (MEMF) for solid-phase microextraction (SPME) of five benzimidazole anthelmintics was prepared by in-situ polymerization. The effect of polymerization conditions on SPME of the target analytes was studied thoroughly. The physicochemical properties of the monolith were characterized by infrared spectroscopy, elemental analysis, scanning electron microscopy, and mercury intrusion porosimetry. Several conditions affecting the extraction efficiency were investigated and, under the optimized conditions, a simple and sensitive method for the determination of trace benzimidazoles residues in milk and honey was established by coupling MEMF-SPME with high-performance liquid chromatography–diode array detection (MEMF-SPME–HPLC–DAD). Under the optimum experimental conditions, the limits of detection (S/N = 3) of the method were 0.11–0.30 μg L−1 for milk and 0.086–0.28 μg L−1 for honey. Evaluation of intra-day and inter-day precision showed reproducibility was satisfactory—relative standard deviations (RSD) for both were <10 %. Finally, the method was successfully used for determination of benzimidazole residues in milk and honey. Recoveries obtained for determination of benzimidazole anthelmintics in spiked samples ranged from 72.3 to 121 %, with RSD always <11 %.

Graphical Abstract

The MEMF-SPME–HPLC–DAD procedure of the determination of benzimidazole anthelmintics in milk and honey samples

Keywords

Monolithic fiber Solid-phase microextraction Benzimidazole anthelmintics Milk Honey 
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Notes

Acknowledgments

The work described in this paper was the supported by National Natural Science Foundation of China (grant 21377105); Fundamental Research Funds for the Central Universities of China (20720140510); Fundamental Innovation Research Funds for postgraduates in Xiamen University (201412G014); and New Century Excellent Talents in Fujian Province University.

Supplementary material

216_2014_8284_MOESM1_ESM.pdf (946 kb)
ESM 1 (PDF 946 kb)

References

  1. 1.
    Balizs G (1999) Determination of benzimidazole residues using liquid chromatography and tandem mass spectrometry. J Chromatogr B 727:167–177CrossRefGoogle Scholar
  2. 2.
    Danaher M, De Ruyck H, Crooks SR, Dowling G, O’Keeffe M (2007) Review of methodology for the determination of benzimidazole residues in biological matrices. J Chromatogr B 845:1–37CrossRefGoogle Scholar
  3. 3.
    Lanusse C, Prichard R (1993) Clinical pharmacokinetics and metabolism of benzimidazole anthelmintics in ruminants. Drug Metab Rev 25:235–279CrossRefGoogle Scholar
  4. 4.
    Zamora O, Paniagua EE, Cacho C, Vera-Avila LE, Perez-Conde C (2009) Determination of benzimidazole fungicides in water samples by on-line MISPE-HPLC. Anal Bioanal Chem 393:1745–1753CrossRefGoogle Scholar
  5. 5.
    Council Regulation of the European Union No. 2377/90 and Commission Regulation Nos. 508/1999, 2385/1999, 2393/1999 and 807/2001Google Scholar
  6. 6.
    Brandon DL, Bates AH, Binder RG, Montague WC, Whitehead LC, Barker SA (2002) Analysis of fenbendazole residues in bovine milk by ELISA. J Agric Food Chem 50:5791–5796CrossRefGoogle Scholar
  7. 7.
    Moreno L, Imperiale F, Mottier L, Alvarez L, Lanusse C (2005) Comparison of milk residue profiles after oral and subcutaneous administration of benzimidazole anthelmintics to dairy cows. Anal Chim Acta 536:91–99CrossRefGoogle Scholar
  8. 8.
    Hu X, Wang J, Feng Y (2010) Determination of benzimidazole residues in edible animal food by polymer monolith microextraction combined with liquid chromatography-mass spectrometry. J Agric Food Chem 58:112–119CrossRefGoogle Scholar
  9. 9.
    Thevis M, Kohler M, Thomas A, Maurer J, Schlorer N, Kamber M, Schanzer W (2008) Determination of benzimidazole- and bicyclic hydantoin-derived selective androgen receptor antagonists and agonists in human urine using LC-MS/MS. Anal Bioanal Chem 391:251–261CrossRefGoogle Scholar
  10. 10.
    Chen D, Tao Y, Liu Z, Liu Z, Huang L, Wang Y, Pan Y, Peng D, Dai M, Yuan Z (2010) Development of a high-performance liquid chromatography method to monitor the residues of benzimidazole in bovine milk. J Chromatogr B 878:2928–2932CrossRefGoogle Scholar
  11. 11.
    Huang XJ, Chen LL, Yuan DX, Luo XB (2011) Preparation, characterization and application of a new stir bar sorptive extraction based on poly(vinylphthalimide-co-N’, N’-methylenebisacrylamide) monolith. J Sep Sci 34:3418–3425CrossRefGoogle Scholar
  12. 12.
    Whelan M, Kinsella B, Furey A, Moloney M, Cantwell H, Lehotay SJ, Danaher M (2010) Determination of anthelmintic drug residues in milk using ultra high performance liquid chromatography-tandem mass spectrometry with rapid polarity switching. J Chromatogr A 1217:4612–4622CrossRefGoogle Scholar
  13. 13.
    Msagati TA, Nindi MM (2001) Determination of benzimidazole compounds by supported liquid membrane extraction and liquid chromatography. J Sep Sci 24:606–614CrossRefGoogle Scholar
  14. 14.
    López Monzón A, Vega Moreno D, Torres Padrón ME, Sosa Ferrera Z, Santana Rodríguez JJ (2007) Solid-phase microextraction of benzimidazole fungicides in environmental liquid samples and HPLC–fluorescence determination. Anal Bioanal Chem 387:1957–1963CrossRefGoogle Scholar
  15. 15.
    Ji YS, Liu XY, Jiang XM, Huang HY, Xu ZG, Zhang HX, Wang CM (2009) Oxidized multiwalled carbon nanotubesas an SPME fiber coating for rapid LC-UV analysis of benzimidazole fungicides in water. Chromatographia 70:753–759CrossRefGoogle Scholar
  16. 16.
    Liu Q, Cheng MT, Long YM, Yu M, Wang T, Jiang GB (2014) Graphenized pencil lead fiber: facile preparation and application in solid-phase microextraction. J Chromatogr A 1325:1–7CrossRefGoogle Scholar
  17. 17.
    Luo Y, Yuan B, Yu Q, Feng YQ (2012) Substrateless grapheme fiber: a sorbent for solid-phase microextraction. J Chromatogr A 1268:9–15CrossRefGoogle Scholar
  18. 18.
    Shi ZG, Chen F, Xing J, Feng YQ (2009) Carbon monolith: preparation, characterization and application as microextraction fiber. J Chromatogr A 1216:5333–5339CrossRefGoogle Scholar
  19. 19.
    Hjertén S, Liao J, Zhang R (1989) High-performance liquid chromatography on continuous polymer beds. J Chromatogr A 473:273–275CrossRefGoogle Scholar
  20. 20.
    Minakuchi H, Nakanishi K, Soga N, Ishizuka N, Tanaka N (1996) Octadecylsilylated porous silica roads as separartion media for reversed-phase liquid chromatography. Anal Chem 68:3498–3505CrossRefGoogle Scholar
  21. 21.
    De Souze Silveira CD, Martendal E, Soldi V, Carasek E (2012) Application of solid-phase microextraction and gas chromatography-mass spectrometry for the determination of chlorophenols in leather. J Sep Sci 35:602–607CrossRefGoogle Scholar
  22. 22.
    Lord H, Pawliszyn J (2000) Microextraction of drugs. J Chromatogr A 902:17–63CrossRefGoogle Scholar
  23. 23.
    Aguilera-luiz MM, Romero-gonzález R, Plaza-bolańos P (2013) Rapid and Semiautomated Method for the Analysis of Veterinary Drug Residues in Honey Based on Turbulent-Flow Liquid Chromatography Coupled to Ultrahigh-Performance Liquid Chromatography–Orbitrap Mass Spectrometry (TFC-UHPLC-Orbitrap-MS). J Agric Food Chem 61:829–839CrossRefGoogle Scholar
  24. 24.
    Su SC, Chang CL, Chang PC, Chou SS (2003) Simultaneous determination of albendazole, thiabendazole, mebendazole and their metabolites in livestock by high performance liquid chromatography. J Food Drug Anal 11:307–319Google Scholar
  25. 25.
    Santaladchaiyakit Y, Srijaranai S (2013) Preconcentration and simultaneous Analysis of Benzimidazole Anthelmintics in Milk Samples by Ultrasound-Assisted Surfactant-Enhanced Emulsification Microextraction and High-Performance Liquid Chromatography. Food Anal Methods 6:1551–1560CrossRefGoogle Scholar
  26. 26.
    De Ruyck H, Daeseleire E, De Ridder H, Van Renterghem R (2002) Development and validation of a liquid chromatogric-electrospray tandem mass spectrometric multiresidue method for anthelmintics in milk. J Chromatogr A 976:181–194CrossRefGoogle Scholar
  27. 27.
    Xia X, Dong Y, Luo P, Wang X, Li X, Ding S, Shen J (2010) Determination of benzimidazole residues in bovine milk by ultra-high performance liquid chromatography-tandem mass spectrometry. J Chromatogr B 878:3174–3180CrossRefGoogle Scholar
  28. 28.
    Msagati TA, Nindi MM (2009) Comparative study of sample preparation methods; supported liquid membrane and solid phase extraction in the determination of benzimidazole anthelmintics in biological matrices by liquid chromatography-electrospray-mass spectrometry. Talanta 69:243–250CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.State Key Laboratory of Marine Environmental Science, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, College of the Environment and EcologyXiamen UniversityXiamenChina

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