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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 1, pp 331–341 | Cite as

On-line stop-flow two-dimensional liquid chromatography–mass spectrometry method for the separation and identification of triterpenoid saponins from ginseng extract

  • Shuangyuan Wang
  • Lizhen Qiao
  • Xianzhe ShiEmail author
  • Chunxiu Hu
  • Hongwei Kong
  • Guowang XuEmail author
Research Paper
Part of the following topical collections:
  1. Multidimensional Chromatography

Abstract

A method based on stop-flow two-dimensional liquid chromatography coupled with electrospray ionization mass spectrometry (2D LC-ESI MS) was established and applied to analyze triterpenoid saponins from the main root of ginseng. Due to the special structure of triterpenoid saponins (they contain polar sugar side chains and nonpolar aglycones), hydrophilic interaction chromatography (HILIC) and reversed-phase liquid chromatography (RPLC) were used for the two dimensions, respectively. A trap column was used to connect the two dimensions. The dilution effect, which is one of the main shortcomings of traditional comprehensive 2D LC methods, was largely avoided. The peak capacity of this system was 747 and the orthogonality was 56.6 %. Compared with one-dimensional HILIC or RP LC MS analysis, 257 and 185 % more mass spectral peaks (ions with intensities that were higher than 1,000) were obtained from the ginseng main root extracts, and 94 triterpenoid saponins were identified based on MSn information and summarized aglycone structures. Given its good linearity and repeatability, the established method was successfully applied to classify ginsengs of different ages (i.e., years of growth), and 19 triterpenoid saponins were found through statistical analysis to vary in concentration depending on the age of the ginseng.

Graphical Abstract

Process of saponin's analysis using stop-flow 2D LC method

Keywords

Stop-flow Two-dimensional liquid chromatography Ginseng Triterpenoid saponins 

Notes

Acknowledgments

The study was supported by the National Basic Research Program (nos. 2012CB720801, 2012CB517506) from the State Ministry of Science and Technology of China, the Foundations (nos. 21175132 and 21275141) and the Creative Research Group Project (no. 21321064) from the National Natural Science Foundation of China.

Supplementary material

216_2014_8219_MOESM1_ESM.pdf (322 kb)
ESM 1 (PDF 321 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Key Laboratory of Separation Science for Analytical ChemistryDalian Institute of Chemical Physics, Chinese Academy of SciencesDalianChina

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