Advertisement

Analytical and Bioanalytical Chemistry

, Volume 406, Issue 30, pp 8037–8040 | Cite as

The nano-bio interface mapped by oxidative footprinting of the adsorption sites of myoglobin

  • Stéphanie Devineau
  • Christelle Mathé
  • Véronique Legros
  • Florence Gonnet
  • Régis Daniel
  • Jean Philippe Renault
  • Serge Pin
Note

Abstract

Oxidative footprinting has been used to study the structure of macromolecular assemblies such as protein-protein and protein-ligand complexes. We propose a novel development of this technique to probe the protein corona that forms at the surface of nanoparticles in any biological medium. Indeed, very few techniques allow studying this interface at the molecular and residue level. Based on hydroxyl radical-mediated oxidation of proteins and analysis by nanoscale liquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS), two sites of adsorption of myoglobin on silica nanoparticles are identified. This method gives new insights in the understanding of protein adsorption on nanomaterials.

Graphical Abstract

The adsorption sites of myoglobin on silica nanoparticles are mapped by oxidative footprinting of the free (a) and adsorbed (b) protein

Keywords

Radical Peptide oxidation Mass spectrometry Nanoparticle Adsorption 

Notes

Acknowledgments

This work was supported in part by C’Nano IdF and Programme Transversal de Toxicologie du CEA.

References

  1. 1.
    Devineau S, Zanotti J-M, Loupiac C, Zargarian L, Neiers F, Pin S, Renault JP (2013) Myoglobin on silica: a case study of the impact of adsorption on protein structure and dynamics. Langmuir 29:13465–13472CrossRefGoogle Scholar
  2. 2.
    Engel M, Visser A, van Mierlo C (2004) Conformation and orientation of a protein folding intermediate trapped by adsorption. PNAS 101:11316–11321CrossRefGoogle Scholar
  3. 3.
    Xu G, Chance MR (2007) Hydroxyl radical-mediated modification of proteins as probes for structural proteomics. Chem Rev 107:3514–3543CrossRefGoogle Scholar
  4. 4.
    Roeser J, Bischoff R, Bruins AP, Permentier HP (2010) Oxidative protein labeling in mass-spectrometry-based proteomics. Anal Bioanal Chem 397:3441–3455CrossRefGoogle Scholar
  5. 5.
    Mathé C, Devineau S, Aude J-C, Lagniel G, Chédin S, Legros V, Mathon M-H, Renault JP, Pin S, Boulard Y, Labarre J (2013) Structural determinants for protein adsorption/non-adsorption to silica surface. PLos ONE 8:1–13CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Stéphanie Devineau
    • 1
    • 4
  • Christelle Mathé
    • 1
    • 5
  • Véronique Legros
    • 2
    • 3
  • Florence Gonnet
    • 2
    • 3
  • Régis Daniel
    • 2
    • 3
  • Jean Philippe Renault
    • 1
  • Serge Pin
    • 1
  1. 1.CEA Saclay, LIDyL, Physico-Chimie sous Rayonnement, UMR 3299 CEA-CNRSGif-sur-Yvette CedexFrance
  2. 2.CNRS, UMR 8587, Laboratoire Analyse et Modélisation pour la Biologie et l’EnvironnementEvry CedexFrance
  3. 3.Laboratoire Analyse et Modélisation pour la Biologie et l’EnvironnementUniversité d’Evry-Val-d’EssonneEvry CedexFrance
  4. 4.Ecole Normale Supérieure-PSL Research University, Department of Chemistry, Sorbonne Universités-UPMC Universite Paris 06, CNRS UMR 8640 PASTEURParisFrance
  5. 5.Université de Lorraine, ENSAIAVandœuvre-lès-NancyFrance

Personalised recommendations