Analytical and Bioanalytical Chemistry

, Volume 406, Issue 29, pp 7729–7739 | Cite as

Haloperidol imprinted polymer: preparation, evaluation, and application for drug assay in brain tissue

  • Aboubakr Rahmani
  • Amir Hooshang Mohammadpour
  • Adeleh Sahebnasagh
  • Seyed Ahmad MohajeriEmail author
Research Paper


Several molecularly imprinted polymers (MIPs) were prepared in the present work, and their binding properties were evaluated in comparison with a nonimprinted polymer (NIP). An optimized MIP was selected and applied for selective extraction and analysis of haloperidol in rabbit brain tissue. A molecularly imprinted solid-phase extraction (MISPE) method was developed for cleanup and preconcentration of haloperidol in brain samples before HPLC-UV analysis. Selectivity of the MISPE procedure was investigated using haloperidol and some structurally different drugs with similar polarity that could exist simultaneously in brain tissue. The extraction and analytical process was calibrated in the range of 0.05–10 ppm. The recovery of haloperidol in this MISPE process was calculated between 79.9 and 90.4 %. The limit of detection (LOD) and the limit of quantification (LOQ) of the assay were 0.008 and 0.05 ppm, respectively. Intraday precision and interday precision values for haloperidol analysis were less than 5.86 and 7.63 %, respectively. The MISPE method could effectively extract and concentrate haloperidol from brain tissue in the presence of clozapine and imipramine. Finally, the imprinted polymer was successfully applied for the determination of haloperidol in a real rabbit brain sample after administration of a toxic dose. Therefore, the proposed MISPE method could be applied in the extraction and preconcentration before HPLC-UV analysis of haloperidol in rabbit brain tissue.


Haloperidol Molecularly imprinted polymer Solid-phase extraction Brain tissue 







Maximum binding sites


Ethylene glycol dimethacrylate


Imprinting factor


Dissociation constant


Methacrylic acid


Molecularly imprinted solid-phase extraction


Nonimprinted polymer



We gratefully acknowledge the Vice Chancellor of Research, Mashhad University of Medical Sciences for financial support through grant number 900160. The results described in this paper were part of a Pharm.D student (Abubakr Rahmani) thesis.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Aboubakr Rahmani
    • 1
  • Amir Hooshang Mohammadpour
    • 2
  • Adeleh Sahebnasagh
    • 1
  • Seyed Ahmad Mohajeri
    • 1
    Email author
  1. 1.Pharmaceutical Research Center, School of PharmacyMashhad University of Medical SciencesMashhadIran
  2. 2.Department of Clinical Pharmacy, Pharmaceutical Research Center and School of PharmacyMashhad University of Medical SciencesMashhadIran

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