Analytical and Bioanalytical Chemistry

, Volume 406, Issue 26, pp 6485–6497 | Cite as

GMO quantification: valuable experience and insights for the future

  • Mojca Milavec
  • David Dobnik
  • Litao Yang
  • Dabing Zhang
  • Kristina Gruden
  • Jana Žel
Review
Part of the following topical collections:
  1. Nucleic Acid Quantification

Abstract

Cultivation and marketing of genetically modified organisms (GMOs) have been unevenly adopted worldwide. To facilitate international trade and to provide information to consumers, labelling requirements have been set up in many countries. Quantitative real-time polymerase chain reaction (qPCR) is currently the method of choice for detection, identification and quantification of GMOs. This has been critically assessed and the requirements for the method performance have been set. Nevertheless, there are challenges that should still be highlighted, such as measuring the quantity and quality of DNA, and determining the qPCR efficiency, possible sequence mismatches, characteristics of taxon-specific genes and appropriate units of measurement, as these remain potential sources of measurement uncertainty. To overcome these problems and to cope with the continuous increase in the number and variety of GMOs, new approaches are needed. Statistical strategies of quantification have already been proposed and expanded with the development of digital PCR. The first attempts have been made to use new generation sequencing also for quantitative purposes, although accurate quantification of the contents of GMOs using this technology is still a challenge for the future, and especially for mixed samples. New approaches are needed also for the quantification of stacks, and for potential quantification of organisms produced by new plant breeding techniques.

Keywords

GMOs Quantification Real-time PCR Digital PCR New generation sequencing 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Mojca Milavec
    • 1
  • David Dobnik
    • 1
  • Litao Yang
    • 2
  • Dabing Zhang
    • 2
  • Kristina Gruden
    • 1
  • Jana Žel
    • 1
  1. 1.Department of Biotechnology and Systems BiologyNational Institute of Biology (NIB)LjubljanaSlovenia
  2. 2.Collaborative Innovation Centre for Biosafety of GMO, National Centre for Molecular Characterisation of GMOs, School of Life Science and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina

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