Analytical and Bioanalytical Chemistry

, Volume 406, Issue 25, pp 6409–6417 | Cite as

A novel and rapid HPGPC-based strategy for quality control of saccharide-dominant herbal materials: Dendrobium officinale, a case study

  • Jun Xu
  • Song-Lin Li
  • Rui-Qi Yue
  • Chun-Hay Ko
  • Jiang-Miao Hu
  • Jing Liu
  • Hing-Man Ho
  • Tao Yi
  • Zhong-Zhen Zhao
  • Jun Zhou
  • Ping-Chung Leung
  • Hu-Biao Chen
  • Quan-Bin Han
Research Paper

Abstract

Qualitative and quantitative characterization of natural saccharides, especially polysaccharides, in herb materials remains a challenge due to their complicated structures and high macromolecular masses. Currently available methods involve time-consuming and complicated operations, and present poor specificity. Here, a novel and rapid high-performance gel permeation chromatography (HPGPC)-based approach is described for quality assessment of saccharide-dominant herbal materials by simultaneous qualitative and quantitative analysis of saccharide components. Dendrobium officinale, one of the rarest tonic herbs worldwide, was employed as the model herb in this study. First, a HPGPC fingerprint based on the molecular weight distribution of its carbohydrate components was established for qualitative identification of D. officinale. Then, HPGPC-guided dominant holistic polysaccharide marker was separated using ultra-filtration followed by HPGPC determination for quantitative evaluation of D. officinale. The experimental results suggest that this method is more efficient, stable, and convenient compared with the currently available methods for authentication and quality evaluation of D. officinale, and we expect the method will have similar advantages when used for quality control of other saccharide-dominant herbal materials and products.

Graphical Abstract

The characteristic HPGPC fingerprint of Dendrobium officinale compared with other confused Dendrobium species

Keywords

HPGPC fingerprints Saccharide-dominant herbal materials Holistic polysaccharide marker Quality evaluation Dendrobium officinale 

Supplementary material

216_2014_8060_MOESM1_ESM.pdf (528 kb)
ESM 1(PDF 527 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Jun Xu
    • 1
  • Song-Lin Li
    • 2
  • Rui-Qi Yue
    • 1
  • Chun-Hay Ko
    • 3
  • Jiang-Miao Hu
    • 4
  • Jing Liu
    • 1
  • Hing-Man Ho
    • 1
  • Tao Yi
    • 1
  • Zhong-Zhen Zhao
    • 1
  • Jun Zhou
    • 4
  • Ping-Chung Leung
    • 3
  • Hu-Biao Chen
    • 1
  • Quan-Bin Han
    • 1
  1. 1.School of Chinese MedicineHong Kong Baptist UniversityHong Kong SARChina
  2. 2.Department of Pharmaceutical Analysis and MetabolomicsJiangsu Branch of China Academy of Chinese Medical Sciences and Jiangsu Province Academy of Traditional Chinese MedicineNanjingChina
  3. 3.State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese MedicineThe Chinese University of Hong KongHong Kong SARChina
  4. 4.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina

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