Analytical and Bioanalytical Chemistry

, Volume 406, Issue 25, pp 6319–6327 | Cite as

Identification of 3-chloro-1,2-propandiol using molecularly imprinted composite solid-phase extraction materials

  • Yun LiEmail author
  • Chuangmu Zheng
  • Xiulan SunEmail author
  • Ben Ouyang
  • Ping Ni
  • Yingzhi Zhang
Research Paper


A novel molecularly imprinted material based on silica microparticles was synthesized by surface polymerization with 3-chloro-1,2-propandiol (3-MCPD) as a template molecule. The molecularly imprinted polymer (MIP) was characterized by infrared spectroscopy and scanning electron microscopy. The adsorption of 3-MCPD by MIP was measured by gas chromatography with electron capture detection (GC-ECD) and an equilibrium binding experiment. Scatchard analysis revealed that the maximum apparent binding capacities of the MIP and non-imprinted polymer (NIP) were 67.64 and 23.31 μmol/g, respectively. The new adsorbent was successfully used in solid-phase extraction (SPE) to selectively enrich and determine 3-MCPD in soy sauce samples. The MIP-SPE column achieves recoveries higher than 92.7 % with a relative standard deviation of less than 1.83 %. The MIP-SPE-GC protocol improved the selectivity and eliminated the effects of template leakage on quantitative analysis and could be used for the determination of 3-MCPD in other complex food samples.

Graphical Abstract

The MIP-SPE column developed by us achieves recoveries higher than 92.7 % with a relative standard deviation of less than 1.83 % for determining the 3-MCPD in the soy sauce matrix (mixed with 3-MCPD, 2-MPCD and 1,3-DCP).


3-MCPD Molecular imprinting Silica Microparticles Adsorption 



The authors are mainly grateful to “973” National Basic Research Program of China (No. 2012CB720804), and the Commonweal Project of the Ministry of Agriculture (No. 201203069-1) for funding the research. This work has also been supported by Program for New Century Excellent Talents in Jiangsu University, Synergetic Innovation Center of Food Safety & Quality Control Jiangsu Province, the Priority Academic Program Development of Jiangsu Higher Education Institutions, and partly supported by “Risk Assessment for Agro-products Quality & Safety” Financial Fund of Ministry of Agriculture.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Key Laboratory of Agro-food Safety and Quality of Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Agro-productsChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and NutritionJiangnan UniversityWuxiPeople’s Republic of China
  3. 3.College of Tea & Food Science and TechnologyAnhui Agricultural UniversityHefeiPeople’s Republic of China

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