Analytical and Bioanalytical Chemistry

, Volume 406, Issue 20, pp 4931–4939 | Cite as

Assessment of ionic liquid stationary phases for the GC analysis of fatty acid methyl esters

  • Katja Dettmer
Research Paper


The gas chromatographic separation of fatty acid methyl esters (FAMEs) on ionic liquid stationary phases was investigated. Seven commercially available ionic liquid columns were tested using a test mixture containing 37 fatty acid methyl esters. The influence of column temperature on the elution order was studied using five different temperature programs. Retention times were highly reproducible. Similar retention behavior was observed for the IL59, IL60, and IL61 columns. The peak pair C18:1 cis/trans was not baseline resolved on these columns, whose stationary phases are highly similar. C18:2 cis/trans, C18:3 n6/n3, and C20:3 n6/n3 were baseline separated on all columns. Baseline separation of the complete test mix was only obtained on the IL82 column using a heating rate of 5 K/min. In general, retention times decreased with increasing column polarity but unsaturated FAMEs were retained stronger compared to their saturated counterparts. Except for the IL59 column, retention crossover was observed when the temperature program was changed.


Ionic liquid columns Gas chromatography Fatty acid methyl esters Retention crossover 



This study was in part funded by KFO 262. The support by Supelco providing the ionic liquid columns is gratefully acknowledged. The technical support by Nadine Nürnberger is highly appreciated.

Supplementary material

216_2014_7919_MOESM1_ESM.pdf (67 kb)
ESM 1 (PDF 66.7 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Institute of Functional GenomicsUniversity of RegensburgRegensburgGermany

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