Analytical and Bioanalytical Chemistry

, Volume 406, Issue 14, pp 3365–3370 | Cite as

A biocatalytic cascade with several output signals—towards biosensors with different levels of confidence

  • Nataliia Guz
  • Jan Halámek
  • James F. Rusling
  • Evgeny Katz
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Abstract

The biocatalytic cascade based on enzyme-catalyzed reactions activated by several biomolecular input signals and producing output signal after each reaction step was developed as an example of a logically reversible information processing system. The model system was designed to mimic the operation of concatenated AND logic gates with optically readable output signals generated at each step of the logic operation. Implications include concurrent bioanalyses and data interpretation for medical diagnostics.

Figure

A biocatalytic cascade with several inputs–outputs was designed for bioanalytical applications providing responses with increasing levels of confidence

Keywords

Bioanalytical methods Bioassays Enzymes Optical sensors Biocomputing Logic gates 
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Notes

Acknowledgments

This work was supported by the NSF award #CBET-1066397 (Clarkson University) and by Grant No. EB014586 (Univ. of CT) from the National Institute of Biomedical Imaging and Bioengineering (NIBIB), NIH.

Supplementary material

216_2014_7789_MOESM1_ESM.pdf (116 kb)
ESM 1 (PDF 116 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Nataliia Guz
    • 1
  • Jan Halámek
    • 2
  • James F. Rusling
    • 3
    • 4
    • 5
  • Evgeny Katz
    • 1
  1. 1.Department of Chemistry and Biomolecular ScienceClarkson UniversityPotsdamUSA
  2. 2.Department of Chemistry, University at AlbanyState University of New YorkAlbanyUSA
  3. 3.Department of Chemistry and Institute of Materials ScienceUniversity of ConnecticutStorrsUSA
  4. 4.School of ChemistryNational University of IrelandGalwayIreland
  5. 5.Department of Cell BiologyUniversity of Connecticut Health CenterFarmingtonUSA

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