Analytical and Bioanalytical Chemistry

, Volume 406, Issue 7, pp 1903–1908

A colorimetric indicator-displacement assay array for selective detection and identification of biological thiols

Research Paper

Abstract

A simple, inexpensive yet highly selective colorimetric indicator-displacement assay array for the simultaneous detection and identification of three important biothiols at micromolar concentrations under physiological conditions and in real samples has been developed in this work. With use of an array composed of metal indicators and metal ions, clear differentiation among cysteine, homocysteine and glutathione was achieved. On the basis of the colour change of the array, quantification of each analyte was accomplished easily, and different biothiols were identified readily using standard chemometric approaches (hierarchical clustering analysis). Moreover, the colorimetric sensor array was not responsive to changes with 19 other natural amino acids, and it showed excellent reproducibility. Importantly, the sensor array developed was successfully applied to the determination and identification of the three biothiols in a real biological sample.

Figure

A simple, inexpensive yet highly selective colorimetric indicator-displacement assay array for the simultaneous detection and identification of three important biothiols was developed in this work

Keywords

Colorimetric sensor array Biothiols Indicator-displacement assay Hierarchical clustering analysis 

Supplementary material

216_2013_7591_MOESM1_ESM.pdf (1.1 mb)
ESM 1(PDF 1139 kb)

References

  1. 1.
    Hong R, Han G, Fernández JM, Kim BJ, Forbes NS, Rotello VM (2006) J Am Chem Soc 128:1078–1079CrossRefGoogle Scholar
  2. 2.
    Wood ZA, Schröder E, Harris JR, Poole LB (2003) Trends Biochem Sci 28:32–40CrossRefGoogle Scholar
  3. 3.
    Weerapana E, Wang C, Simon GM, Richter F, Khare S, Dillon MBD, Bachovchin DA, Mowen K, Baker D, Cravatt BF (2010) Nature 468:790–795CrossRefGoogle Scholar
  4. 4.
    Lu SC (2009) Mol Aspects Med 30:42–59CrossRefGoogle Scholar
  5. 5.
    Townsend DM, Tew KD, Tapiero H (2003) Biomed Pharmacother 57:145–155CrossRefGoogle Scholar
  6. 6.
    Refsum H, Ueland PM, Nygard O, Vollset SE (1998) Annu Rev Med 49:31–62CrossRefGoogle Scholar
  7. 7.
    Seshadri S, Beiser A, Selhub J, Jacques PF, Rosenberg IH, D’Agostino RB, Wilson PWF, Wolf PA (2002) N Engl J Med 346:476–483CrossRefGoogle Scholar
  8. 8.
    Özyürek M, Baki S, Güngör N, Çelik SE, Güçlü K, Apak R (2012) Anal Chim Acta 750:173–181CrossRefGoogle Scholar
  9. 9.
    Jin W, Wang Y (1997) J Chromatogr A 769:307–314CrossRefGoogle Scholar
  10. 10.
    Safavi A, Maleki N, Farjami E, Mahyari FA (2009) Anal Chem 81:7538–7543CrossRefGoogle Scholar
  11. 11.
    Gao Y, Li Y, Zou X, Huang H, Su XG (2012) Anal Chim Acta 731:68–74CrossRefGoogle Scholar
  12. 12.
    Güçlü K, Özyürek M, Güngör N, Baki S, Apak R (2013) Anal Chim Acta 794:90–98CrossRefGoogle Scholar
  13. 13.
    Chen X, Ko SK, Kim MJ, Shin I, Yoon J (2010) Chem Commun 46:2751–2753CrossRefGoogle Scholar
  14. 14.
    Moragues ME, Martinez-Manez R, Sancenon F (2011) Chem Soc Rev 40:2593–2643CrossRefGoogle Scholar
  15. 15.
    Yao Z, Bai H, Li C, Shi G (2011) Chem Commun 47:7431–7433CrossRefGoogle Scholar
  16. 16.
    Niu LY, Guan YS, Chen YZ, Wu LZ, Tung CH, Yang QZ (2012) J Am Chem Soc 134:18928–18931CrossRefGoogle Scholar
  17. 17.
    Yuan X, Tay YQ, Dou XY, Luo ZT, Leong DT, Xie JP (2013) Anal Chem 85:1913–1919CrossRefGoogle Scholar
  18. 18.
    Severin K (2010) Curr Opin Chem Bio 14:737–742CrossRefGoogle Scholar
  19. 19.
    Rakow NA, Suslick KS (2000) Nature 406:710–713CrossRefGoogle Scholar
  20. 20.
    Rochat S, Severin K (2010) J Comb Chem 12:595–599CrossRefGoogle Scholar
  21. 21.
    Collins BE, Anslyn EV (2007) Chem Eur J 13:4700–4708CrossRefGoogle Scholar
  22. 22.
    Buryak A, Pozdnoukhov A, Severin K (2007) Chem Commun 2366–2368.Google Scholar
  23. 23.
    Kitamura M, Shabbir SH, Anslyn EV (2009) J Org Chem 74:4479–4489CrossRefGoogle Scholar
  24. 24.
    Albert KJ, Lewis NS, Schauer CL, Sotzing GA, Stitzel SE, Vaid TP, Walt DR (2000) Chem Rev 100:2595–2626CrossRefGoogle Scholar
  25. 25.
    Corbett PT, Leclaire J, Vial L, West KR, Wietor JL, Sanders JKM, Otto S (2006) Chem Rev 106:3652–3711CrossRefGoogle Scholar
  26. 26.
    Wiskur SL, Ait-Haddou H, Lavigne JJ, Anslyn EV (2001) Acc Chem Res 34:963–972CrossRefGoogle Scholar
  27. 27.
    Lim SH, Feng L, Kemling JW, Musto CJ, Suslick KS (2009) Nat Chem 1:562–567CrossRefGoogle Scholar
  28. 28.
    Niu LY, Li H, Feng L, Guan YS, Chen YZ, Duan CF, Wu LZ, Guan YF, Tung CH, Yang QZ (2013) Anal Chim Acta 775:93–99CrossRefGoogle Scholar
  29. 29.
    Feng L, Li X, Li H, Yang W, Chen L, Guan YF (2013) Anal Chim Acta 780:74–80CrossRefGoogle Scholar
  30. 30.
    Schiller A, Vilozny B, Wessling RA, Singaram B (2008) Anal Chim Acta 627:203–211CrossRefGoogle Scholar
  31. 31.
    Thetea AR, Henkel T, Göckeritz R, Endlich M, Köhler JM, Groß GA (2009) Anal Chim Acta 633:81–89CrossRefGoogle Scholar
  32. 32.
    Zarzo M (2007) Biol Rev 82:455–479CrossRefGoogle Scholar
  33. 33.
    Wang J, Luthey-Schulten ZA, Suslick KS (2003) Proc Natl Acad Sci USA 100:3035–3039CrossRefGoogle Scholar
  34. 34.
    You CC, Miranda OR, Gider B, Ghoash PS, Kim IB, Erdogan B, Krovi SA, Bunz UHF, Rotello VM (2007) Nat Nanotechnol 2:318–323CrossRefGoogle Scholar
  35. 35.
    Nguyen BT, Anslyn EV (2006) Coord Chem Rev 250:3118–3127CrossRefGoogle Scholar
  36. 36.
    Buryak A, Severin K (2006) J Comb Chem 8:540–543CrossRefGoogle Scholar
  37. 37.
    Buryak A, Severin K (2005) Angew Chem Int Ed 44:7935–7938CrossRefGoogle Scholar
  38. 38.
    Metzger A, Anslyn EV (1998) Angew Chem Int Ed 37:649–652CrossRefGoogle Scholar
  39. 39.
    Kumar V, Anslyn EV (2013) J Am Chem Soc 135:6338–6344CrossRefGoogle Scholar
  40. 40.
    Buryak A, Severin K (2005) J Am Chem Soc 127:3700–3701CrossRefGoogle Scholar
  41. 41.
    Buryak A, Zaubitzer F, Poznoukhov A, Severin K (2008) J Am Chem Soc 130:11260–11261CrossRefGoogle Scholar
  42. 42.
    Feng L, Li H, Li X, Chen L, Shen Z, Guan YF (2012) Anal Chim Acta 743:1–8CrossRefGoogle Scholar
  43. 43.
    Wiskur SL, Floriano PN, Anslyn EV, McDevitt JT (2003) Angew Chem Int Ed 42:2070–2072CrossRefGoogle Scholar
  44. 44.
    Pezzato C, Lee B, Severin K, Prins LJ (2013) Chem Commun 49:469–471CrossRefGoogle Scholar
  45. 45.
    Johnson RA, Wichern DW (2007) Applied multivariate statistical analysis, 6th edn. Prentice Hall, Upper Saddle RiverGoogle Scholar
  46. 46.
    Hair JF, Black B, Babin B, Anderson RE, Tatham RL (2005) Multivariate data analysis, 6th edn. Prentice Hall, Upper Saddle RiverGoogle Scholar
  47. 47.
    Tan HL, Chen Y (2012) J Biomed Opt 17:17001–17007CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingboChina

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