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Analytical and Bioanalytical Chemistry

, Volume 406, Issue 5, pp 1551–1556 | Cite as

Molecularly imprinted solid-phase extraction monolithic capillary column for selective extraction and sensitive determination of safranine T in wolfberry

  • Zihao Su
  • Haiyun ZhaiEmail author
  • Zuanguang ChenEmail author
  • Qing Zhou
  • Jiangmei Li
  • Zhenping Liu
Research Paper

Abstract

A method was developed to sensitively determine safranine T in wolfberry by molecularly imprinted solid-phase extraction (MISPE) coupled with high-performance liquid chromatography and laser-induced fluorescence detection (HPLC-LIF). The MISPE capillary monolithic column was prepared by water-bath in situ polymerization, using safranine T, methacrylic acid (MAA), and ethylene dimethacrylate (EDMA) as template, functional monomer, and cross-linker, respectively. The properties of the homemade MISPE capillary monolithic column, including capacity and specificity, were investigated under optimized conditions and the morphologies of inner polymers were characterized by scanning electron microscopy (SEM). The mean recoveries of safranine T in wolfberry ranged from 91.2 % to 92.9 % and the intraday and interday relative standard deviation (RSD) values all ranged from 3.4 % to 4.2 %. Good linearity was obtained over 0.001–1.0 μg mL–1 (r = 0.9999) with a detection limit (S/N = 3) of 0.4 ng g–1. Under the selected conditions, enrichment factors of over 90-fold were obtained and the extraction on the monolithic column effectively cleaned up the wolfberry matrix. The results demonstrated that the proposed MISPE-HPLC-LIF method could be applied to sensitively determine safranine T in wolfberry.

Figure

SEM images of the monolithic column prepared with different initiation reaction methods: a UV initiation; b water bath; c 5000-folds magnification of b; d 20000-folds magnification of b

Keywords

Safranine T MIPs SPE In situ polymerization Monolithic capillary column Wolfberry 

Notes

Acknowledgments

The authors gratefully acknowledge financial support from the National Natural Science Foundation of China (NSFC, Grant No. 21005021) and Guangdong Provincial Science and Technology Project (No. 2011112, 2010B020316010).

Supplementary material

216_2013_7541_MOESM1_ESM.pdf (98 kb)
ESM 1 (PDF 97.8 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.College of PharmacyGuangdong Pharmaceutical UniversityGuangzhouChina
  2. 2.School of Pharmaceutical ScienceSun Yat-sen UniversityGuangzhouChina

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