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Analytical and Bioanalytical Chemistry

, Volume 406, Issue 5, pp 1531–1540 | Cite as

A solid-phase fluorescent biosensor for the determination of phenolic compounds and peroxides in samples with complex matrices

  • P. V. Rodionov
  • I. A. VeselovaEmail author
  • T. N. Shekhovtsova
Research Paper

Abstract

A solid-phase fluorescent biosensor for the determination of phenolic compounds (simple substituted phenols and catecholamines) and peroxides has been developed. The biosensor has a simple construction and the analytical signal is measured directly in a biosensitive layer {peroxidase-chitosan} on the sensor surface. This approach allowed analyzing samples with complex matrices (including water-insoluble samples and nontransparent solutions) without their preliminary pretreatment. Two novel fluorescent indicator reactions for the determination of the above-mentioned analytes in wide concentration ranges (from nmol l−1 to mm l−1) which provided an analytical signal registration on a solid phase were proposed. The developed sensor was applied successfully for the analysis of urine, cosmetics, pharmaceuticals preparations, etc.

Keywords

Solid-phase fluorescence biosensor Phenols Catecholamines Peroxides 

Notes

Acknowledgments

This investigation was done with the financial support of Russian Foundation for Basic Research (project 12-03-00249-a) and Russian Federation Ministry of Education and Science (contracts 8425 and 8448, 31.08.2012).

Supplementary material

216_2013_7538_MOESM1_ESM.pdf (483 kb)
ESM 1 (PDF 483 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • P. V. Rodionov
    • 1
  • I. A. Veselova
    • 1
    Email author
  • T. N. Shekhovtsova
    • 1
  1. 1.Chemical DepartmentThe Lomonosov Moscow State UniversityMoscowRussia

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