Analytical and Bioanalytical Chemistry

, Volume 406, Issue 5, pp 1531–1540 | Cite as

A solid-phase fluorescent biosensor for the determination of phenolic compounds and peroxides in samples with complex matrices

  • P. V. Rodionov
  • I. A. VeselovaEmail author
  • T. N. Shekhovtsova
Research Paper


A solid-phase fluorescent biosensor for the determination of phenolic compounds (simple substituted phenols and catecholamines) and peroxides has been developed. The biosensor has a simple construction and the analytical signal is measured directly in a biosensitive layer {peroxidase-chitosan} on the sensor surface. This approach allowed analyzing samples with complex matrices (including water-insoluble samples and nontransparent solutions) without their preliminary pretreatment. Two novel fluorescent indicator reactions for the determination of the above-mentioned analytes in wide concentration ranges (from nmol l−1 to mm l−1) which provided an analytical signal registration on a solid phase were proposed. The developed sensor was applied successfully for the analysis of urine, cosmetics, pharmaceuticals preparations, etc.


Solid-phase fluorescence biosensor Phenols Catecholamines Peroxides 



This investigation was done with the financial support of Russian Foundation for Basic Research (project 12-03-00249-a) and Russian Federation Ministry of Education and Science (contracts 8425 and 8448, 31.08.2012).

Supplementary material

216_2013_7538_MOESM1_ESM.pdf (483 kb)
ESM 1 (PDF 483 kb)


  1. 1.
    Sanz V, Galban J, Marcos S, Castillo JR (2003) Talanta 60:415–423CrossRefGoogle Scholar
  2. 2.
    Scavetta E, Ballarin B, Tonneli D (2010) Electroanalysis 4:427–432CrossRefGoogle Scholar
  3. 3.
    Zhu X, Li C, Zhu X, Xu M (2012) Int J Electroche Sci 7:8522–8532Google Scholar
  4. 4.
    Abdullah J, Ahmad M, Heng LY, Karuppiah N, Sidek H (2006) Anal Bioanal Chem 386:1285–1292CrossRefGoogle Scholar
  5. 5.
    Huang J, Fang H, Liu C, Gu E, Jiang D (2008) Anal Lett 41:1430–1442CrossRefGoogle Scholar
  6. 6.
    Apak R, Cekic SD, Cetinkaya A, Filik H, Hayvali M, Kilic E (2012) J Agric Food Chem 60:2769–2777CrossRefGoogle Scholar
  7. 7.
    Yang RH, Wang KM, Long LP, Chan WH, Yang XH (2002) Analyst 127:119–124CrossRefGoogle Scholar
  8. 8.
    Ni R, Tong RB, Guo CC, Shen GL, Yu RQ (2004) Talanta 63:251–257CrossRefGoogle Scholar
  9. 9.
    Wang X, Zeng H, Wei Y, Lin JM (2006) Sens and Act B 114:565–572CrossRefGoogle Scholar
  10. 10.
    Veselova IA, Malinina LI, Rodionov PV, Shekhovtsova TN (2012) Talanta 102:101–109CrossRefGoogle Scholar
  11. 11.
    Tallury P, Kar S, Bamrungsap S, Huang YF, Tan WH, Santra S (2009) Chem Commun 17:2347–2349CrossRefGoogle Scholar
  12. 12.
    Ma O, Lavertu M, Sun J, Nguyen S, Buschmann MD, Winnik FM, Hoemann CD (2008) Carbohydr Polym 72:616–624CrossRefGoogle Scholar
  13. 13.
    Drexhage KH (1976) J Res 80:421–428Google Scholar
  14. 14.
    Park SA, Jang E, Koh WG, Kim B (2010) Sens and Act B 150:120–125CrossRefGoogle Scholar
  15. 15.
    Yan J, Quan G, Chil J (2009) Chem Soc 54:73–76Google Scholar
  16. 16.
    Li JM, Meng XG, Hu CW, Du J (2009) Bioresour Technol 100:1168–1173CrossRefGoogle Scholar
  17. 17.
    Weinryb I (1966) Biochemistry 5:2003–2008CrossRefGoogle Scholar
  18. 18.
    Paine GF, Raghavan SR (2007) Soft Matter 3:521–527CrossRefGoogle Scholar
  19. 19.
    Temocin Z, Yigitoglu M (2009) Bioproc Biosyst Eng 32:467–474CrossRefGoogle Scholar
  20. 20.
    Kurniavati S, Nicell J (2008) Bioresour Technol 99:7825–7834CrossRefGoogle Scholar
  21. 21.
    Garca-Molina F, Hiner ANP, Fenoll LG, Rodrigues-Lopez JN, Garca-Ruiz PA, Garca-Cnovac F, Tudela J (2005) J Agric Food Chem 53:3702–3709CrossRefGoogle Scholar
  22. 22.
    Mayer AM (1959) Experientia 15:158CrossRefGoogle Scholar
  23. 23.
    Ma Y, Yang C, Li N, Yang X (2005) Talanta 67:979–983CrossRefGoogle Scholar
  24. 24.
    Zhao L, Lv B, Yuan H, Zhou Z, Xiao D (2007) Sensors 7:578–588CrossRefGoogle Scholar
  25. 25.
    Suresh S, Srivastava VC, Mishra IM (2011) J Chem Eng Data 56:811–818CrossRefGoogle Scholar
  26. 26.
    Blanco-Martinez DA, Giraldo L, Moreno-Pirajan JC (2009) J Hazard Mater 169:291–296CrossRefGoogle Scholar
  27. 27.
    Gregg DC, Nelson JM (1940) J Am Chem Soc 62:2510–2512CrossRefGoogle Scholar
  28. 28.
    Tsunoda M (2006) Anal Bioanal Chem 386:506–514CrossRefGoogle Scholar
  29. 29.
    Chang HT, Yeung ES (1995) Anal Chem 67:1079–1083CrossRefGoogle Scholar
  30. 30.
    Takezawa K, Tsunoda M, Murayama K, Santa T, Imai K (2000) Analyst 125:293–296CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • P. V. Rodionov
    • 1
  • I. A. Veselova
    • 1
    Email author
  • T. N. Shekhovtsova
    • 1
  1. 1.Chemical DepartmentThe Lomonosov Moscow State UniversityMoscowRussia

Personalised recommendations