Preparation of magnetic dummy molecularly imprinted polymers for selective extraction and analysis of salicylic acid in Actinidia chinensis
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Compounds with strong intramolecular hydrogen bonds (e.g., salicylic acid) have weak intermolecular hydrogen bonding interactions between them and functional monomers in the imprinting process. Consequently, the corresponding molecularly imprinted polymers (MIPs) have no specific adsorption ability. Here, the first magnetic dummy MIPs (MDMIPs) based on benzonic acid as dummy template are successfully developed and evaluated with respect to the applications in selective enrichment and analysis of salicylic acid from complex mixtures. Various parameters affecting absorption/desorption were evaluated for achieving optimal recovery and reducing nonspecific interactions. The prepared MDMIPs showed high adsorption capacity, good selectivity, rapid kinetic binding (40 min) and magnetic separation (5 s), high reproducibility (RSD< 4 % for batch-to-batch evaluation), and stability (only 4 % decrease after 6 cycles). Owing to the efficacy in specific binding and removal of interference, trace level salicylic acid was quantified (0.2 μg/g of fresh mass) in Actinidia chinensis by high-performance liquid chromatography.
KeywordsMolecularly imprinted polymers Magnetic separation Salicylic acid Actinidia chinensis
This project was supported by the National Natural Science Foundation of China (21275163, 21005089), the Science and Technology Program of Hunan Province, China (2012FJ2006), the Postdoctoral Science Foundation of Central South University, the Shenghua Yuying project of Central South University, and aid program for Science and Technology Innovative Research Team (Chemicals of Forestry Resources and Development of Forest Products) in Higher Educational Institutions of Hunan Province.
- 4.Maldim IM, Souza MCD, Souza JCJD, Figueiredo EC, Martins I (2012) Application of the molecularly imprinted solid-phase extraction to the organophosphate residues determination in strawberries. Anal Bioanal Chem 404:6–7Google Scholar
- 10.Xiao DL, Dramou P, Xiong NQ, He H, Yuan DH, Dai H, Li H, He XM, Peng J, Li N (2013) Preparation of molecularly imprinted polymers on the surface of magnetic carbon nanotubes with a pseudo template for rapid simultaneous extraction of four fluoroquinolones in egg samples. Analyst 138:3287–3296CrossRefGoogle Scholar
- 16.Koeber R, Fleischer C, Lanza F, Boos KS, Sellergren B, Barceló D (2001) Evaluation of a multidimensional solid-phase extraction platform for highly selective on-line cleanup and high-throughput LC-MS analysis of triazines in river water samples using molecularly imprinted polymers. Anal Chem 73:2437–2444CrossRefGoogle Scholar
- 19.Quaglia M, Chenon K, Hall J, De Lorenzi E, Sellergren B (2001) Target analogue imprinted polymers with affinity for folic acid and related compounds. J Am Chem Soc 123:2146–2154Google Scholar
- 22.Raskin I (1992) Role of salicylic acid in plants. Plant Mol Biol 43:439–463Google Scholar
- 42.Zhang Y, Chen KS, Zhang SL (2004) Extraction and determination of endogenous salicylic acid from kiwifruit and its application to postharvest research. J Chin Inst Food Sci Technol 4:6–9Google Scholar