Analytical and Bioanalytical Chemistry

, Volume 406, Issue 3, pp 757–770

Development and validation of a rapid multiplex ELISA for pyrrolizidine alkaloids and their N-oxides in honey and feed

  • Michalina Oplatowska
  • Christopher T. Elliott
  • Anne-Catherine Huet
  • Mark McCarthy
  • Patrick P. J. Mulder
  • Christoph von Holst
  • Philippe Delahaut
  • Hans P. Van Egmond
  • Katrina Campbell
Research Paper
  • 667 Downloads
Part of the following topical collections:
  1. Rapid Detection in Food and Feed

Abstract

Pyrrolizidine alkaloids (PAs) are a group of plant secondary metabolites with carcinogenic and hepatotoxic properties. When PA-producing plants contaminate crops, toxins can be transferred through the food chain and cause illness in humans and animals, most notably hepatic veno-occlusive disease. Honey has been identified as a direct risk of human exposure. The European Food Safety Authority has recently identified four groups of PAs that are of particular importance for food and feed: senecionine-type, lycopsamine-type, heliotrine-type and monocrotaline-type. Liquid or gas chromatography methods are currently used to detect PAs but there are no rapid screening assays available commercially. Therefore, the aim of this study was to develop a rapid multiplex ELISA test for the representatives of three groups of alkaloids (senecionine, lycopsamine and heliotrine types) that would be used as a risk-management tool for the screening of these toxic compounds in food and feed. The method was validated for honey and feed matrices and was demonstrated to have a detection capability less than 25 μg/kg for jacobine, lycopsamine, heliotrine and senecionine. The zinc reduction step introduced to the extraction procedure allows for the additional detection of the presence of N-oxides of PAs. This first multiplex immunoassay for PA detection with N-oxide reduction can be used for the simultaneous screening of 21 samples for >12 PA analytes. Honey samples (n = 146) from various origins were analysed for PA determination. Six samples were determined to contain measurable PAs >25 μg/kg by ELISA which correlated to >10 μg/kg by LC-MS/MS.

Keywords

Multiplex ELISA Pyrrolizidine alkaloids Food and feed Honey Screening assay 

Supplementary material

216_2013_7488_MOESM1_ESM.pdf (95 kb)
ESM 1(PDF 302 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Michalina Oplatowska
    • 1
  • Christopher T. Elliott
    • 1
  • Anne-Catherine Huet
    • 2
  • Mark McCarthy
    • 1
  • Patrick P. J. Mulder
    • 3
  • Christoph von Holst
    • 4
  • Philippe Delahaut
    • 2
  • Hans P. Van Egmond
    • 3
  • Katrina Campbell
    • 1
  1. 1.Institute for Global Food Security, School of Biological SciencesQueen’s University BelfastBelfastUK
  2. 2.Département SantéCentre d’Economie Rurale (CER Groupe)MarloieBelgium
  3. 3.RIKILT-Institute of Food SafetyWageningen URWageningenThe Netherlands
  4. 4.European Commission, DG Joint Research CentreInstitute for Reference Materials and MeasurementsGeelBelgium

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