Change of fucosylated IgG2 Fc-glycoforms in pancreatitis and pancreatic adenocarcinoma: a promising disease-classification model
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The fixed constant (Fc) region of IgG is subject to changes in glycosylation state in response to diseases. On the basis of sera from 75 healthy controls, 75 pancreatitis (PT) patients, and 75 pancreatic adenocarcinoma (PAC) patients, we analyzed six fucosylated glycoforms of IgG2 (G0F, G1F, G2F, G0FN, G1FN, and G2FN), by matrix-assisted laser desorption/ionization–Fourier-transform ion cyclotron resonance mass spectrometry (MALDI–FTICR MS), to evaluate their use as biomarkers for pancreatic diseases. Compared with healthy controls, significant increases in agalactosylated glycoforms and decreases in galactosylated glycoforms were observed for PT and PAC patients. Logistic regression analysis suggested that truncation of the sugar chain was prone to occur in PT and, especially, PAC patients. After participants were stratified by sex and age, receiver operating characteristic curve analysis revealed good overall sensitivity and specificity for discrimination of PAC and PT patients from healthy controls. A combination of G0F and galactosylation also had acceptable power for differentiating PAC patients from PT patients.
KeywordsIgG2 N-glycosylation Pancreatitis Pancreatic adenocarcinoma Diagnosis MALDI–FTICR MS
One-way analysis of variance
Area under receiver operating characteristic curve
- MALDI–FTICR MS
Matrix-assisted laser desorption/ionization–Fourier-transform ion cyclotron resonance mass spectrometry
Receiver operating characteristic
This study was supported by grant no. 21075137 from the National Natural Science Foundation of China and by the Research Fund for the Doctoral Program of Higher Education (grant no. 20121106110023) (to Z. Li).
- 10.Nouts A, Levy P, Voitot H, Bernades P (1998) Diagnostic value of serum Ca 19–9 antigen in chronic pancreatitis and pancreatic adenocarcinoma. Gastroenterol Clin Biol 22:152–159Google Scholar