Analytical and Bioanalytical Chemistry

, Volume 405, Issue 23, pp 7541–7544 | Cite as

A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue

  • Xiaohu Xia
  • Ye Xu
  • Rongqin Ke
  • Heng Zhang
  • Mingqiang Zou
  • Wei Yang
  • Qingge Li


A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol residue was developed. The detection result could be either qualitatively resolved with naked eye or quantitatively analyzed with the assistance of a digital camera. In the qualitative mode, the limit of detection (LOD) was found to be 0.25 ng/mL. In the quantitative mode, the half-maximal inhibition concentration (IC50) was determined to be 0.45 ng/mL and the LOD can reach an ultralow level of 0.03 ng/mL, which is ~100 times lower than that of the conventional colloidal gold-based lateral flow immunoassay. Potential application of the established method was demonstrated by analyzing representative cow milk samples.


A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol (CAP) residue was developed, of which limit of detections (LODs) can reach low levels of 0.25 and 0.03 ng/mL, respectively, in the qualitative and quantitative modes.


Detection Chloramphenicol Lateral flow immunoassay Europium chelate Nanoparticle 



The authors acknowledge financial support for this work by the Commonweal Scientific Foundation for Industry of Chinese Inspection and Quarantine (no. 201010022), the Xiamen Scientific Development Program (no. 3502Z20055008), the National Natural Science Foundation of China (no. 30500454), and the Natural Science Foundation of Fujian Province of China (no. 2007 J0112).

Supplementary material

216_2013_7210_MOESM1_ESM.pdf (9.1 mb)
ESM 1 (PDF 9318 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Xiaohu Xia
    • 1
    • 2
  • Ye Xu
    • 1
  • Rongqin Ke
    • 1
  • Heng Zhang
    • 3
  • Mingqiang Zou
    • 4
  • Wei Yang
    • 1
  • Qingge Li
    • 1
  1. 1.Engineering Research Centre of Molecular Diagnostics, Ministry of Education; Department of Biomedical Sciences, School of Life SciencesXiamen UniversityXiamenChina
  2. 2.The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of MedicineGeorgia Institute of TechnologyAtlantaUSA
  3. 3.Shenzhen Entry-Exit Inspection and Quarantine BureauShenzhenChina
  4. 4.Chinese Academy of Inspection and QuarantineBeijingChina

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