Analytical and Bioanalytical Chemistry

, Volume 405, Issue 23, pp 7321–7335 | Cite as

Profiling and sequence analysis of gangliosides in human astrocytoma by high-resolution mass spectrometry

  • Alina D. ZamfirEmail author
  • Dragana Fabris
  • Florina Capitan
  • Cristian Munteanu
  • Željka Vukelić
  • Corina FlangeaEmail author
Research Paper


In this preliminary investigation, a low-grade astrocytoma (AcT) is investigated by high-resolution (HR) mass spectrometry (MS) aiming at characterization of gangliosides with potential biomarker value. The research was conducted towards a comparative mapping of ganglioside expression in AcT, its surrounding tissue (ST) and a normal control brain tissue (NT). HR MS was conducted in the negative ion mode nanoelectrospray ionization (nanoESI). Fragmentation analysis was carried out by collision-induced dissociation (CID) MS2–MS4. Due to the high resolving power and mass accuracy, by comparative mapping of the ganglioside extracts from AcT, ST and NT, under identical conditions, 37 different species in AcT, 40 in ST and 56 in NT were identified. AcT and ST were found to contain 18 identical ganglioside components. Among all three specimens, ST extract presented the highest levels of sialylation, fucosylation and acetylation, a feature which might be correlated to the tumor expansion in the adjacent brain area. MS mapping indicated also that AcT, ST and NT share one doubly deprotonated molecule at m/z 1063.31, attributable to GT1(d18:1/18:0) or GT1(d18:0/18:1). CID MS2–MS4 on these particular ions detected in AcT and ST provided data supporting GT1c isomer in the investigated astrocytoma tissue. Our results show that HR MS has a remarkable potential in brain cancer research for the determination of tumor-associated markers and for their structural determination.


Ganglioside isomer discrimination in human astrocytoma by Orbitrap multistage MS


Astrocytoma High-resolution mass spectrometry Gangliosides Biomarker Screening and sequencing 





Capillary electrophoresis




Collision-induced dissociation


Electrospray ionization












High-capacity ion trap


High-performance liquid chromatography


High-performance thin layer chromatography


High resolution


Liquid chromatography


Long chain base


Matrix-assisted laser desorption/ionization


Mass spectrometry


Tandem mass spectrometry


Multistage mass spectrometry


Nanoelectrospray ionization


N-acetyl neuraminic acid


Normal brain tissue


Quadrupole time-of-flight


Surrounding tissue


Total ion chromatogram


Thin layer chromatography


World Health Organization



This work was supported by the Romanian National Authority for Scientific Research, CNCS-UEFISCDI, projects PN-II-ID-PCE-2011-3-0047, PN-II-PCCA-2011-142, PN-II-RU-TE-2011-2-0008 and FP7 Marie Curie-PIRSES-GA-2010-269256.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Alina D. Zamfir
    • 1
    • 2
    Email author
  • Dragana Fabris
    • 3
  • Florina Capitan
    • 1
  • Cristian Munteanu
    • 4
  • Željka Vukelić
    • 3
  • Corina Flangea
    • 1
    • 4
    Email author
  1. 1.Department of Chemical and Biological Sciences“Aurel Vlaicu” University of AradAradRomania
  2. 2.Mass Spectrometry LaboratoryNational Institute for Research and Development in Electrochemistry and Condensed MatterTimisoaraRomania
  3. 3.Department of Chemistry and Biochemistry, Faculty of MedicineUniversity of ZagrebZagrebCroatia
  4. 4.Department of Molecular and Cell BiologyBiochemistry Institute of the Romanian AcademyBucharestRomania

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