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Analytical and Bioanalytical Chemistry

, Volume 405, Issue 27, pp 8701–8707 | Cite as

The role of asbestos morphology on their cellular toxicity: an in vitro 3D Raman/Rayleigh imaging study

  • Yao Seydou
  • Hsiang-Hsin Chen
  • Etienne Harte
  • Giancarlo Della Ventura
  • Cyril PetiboisEmail author
Research Paper
Part of the following topical collections:
  1. Morpho-Spectral Imaging

Abstract

Amphiboles caused cohorts of deaths in exposed workers, leading to some of the largest class actions in the industry. Once inhaled, these inorganic fibers are thought to be both chemically and morphologically toxic, and their biopersistence in the lungs over decades lead to progressive pathologies, mesothelioma, and asbestosis. However, this exceptionally long chronicity for human pathologies suggests that chemical toxicity is certainly low, suggesting that morphological parameters could be more relevant in the pathology. Here, we developed a 3D Raman/optical imaging methodology in vitro to characterize both morphological and chemical parameters of cell/fiber interactions. We determined that lung cells could vesiculate amphiboles with length below 5 μm or could embed those not exceeding 15 μm in their fibrous extracellular matrix. Lung cells can thus develop defense strategies for handling the biopersistence of inorganic species, which may thus have major impact for biosafety issues related to nanomaterials.

Keywords

Bioanalytical methods Cell systems/single cell analysis Nanoparticles/nanotechnology Raman imaging Asbestos Inorganic compounds/trace inorganic compounds 

Supplementary material

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Yao Seydou
    • 1
  • Hsiang-Hsin Chen
    • 1
  • Etienne Harte
    • 1
  • Giancarlo Della Ventura
    • 2
  • Cyril Petibois
    • 1
    Email author
  1. 1.Université de Bordeaux, Inserm U1029Talence-CedexFrance
  2. 2.Dipartimento Scienze GeologicheUniversità Roma TreRomeItaly

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