Analytical and Bioanalytical Chemistry

, Volume 405, Issue 27, pp 8701–8707 | Cite as

The role of asbestos morphology on their cellular toxicity: an in vitro 3D Raman/Rayleigh imaging study

  • Yao Seydou
  • Hsiang-Hsin Chen
  • Etienne Harte
  • Giancarlo Della Ventura
  • Cyril PetiboisEmail author
Research Paper
Part of the following topical collections:
  1. Morpho-Spectral Imaging


Amphiboles caused cohorts of deaths in exposed workers, leading to some of the largest class actions in the industry. Once inhaled, these inorganic fibers are thought to be both chemically and morphologically toxic, and their biopersistence in the lungs over decades lead to progressive pathologies, mesothelioma, and asbestosis. However, this exceptionally long chronicity for human pathologies suggests that chemical toxicity is certainly low, suggesting that morphological parameters could be more relevant in the pathology. Here, we developed a 3D Raman/optical imaging methodology in vitro to characterize both morphological and chemical parameters of cell/fiber interactions. We determined that lung cells could vesiculate amphiboles with length below 5 μm or could embed those not exceeding 15 μm in their fibrous extracellular matrix. Lung cells can thus develop defense strategies for handling the biopersistence of inorganic species, which may thus have major impact for biosafety issues related to nanomaterials.


Bioanalytical methods Cell systems/single cell analysis Nanoparticles/nanotechnology Raman imaging Asbestos Inorganic compounds/trace inorganic compounds 

Supplementary material


(WMV 6755 kb)


(WMV 4428 kb)


(AVI 6421 kb)

216_2013_7143_MOESM4_ESM.pdf (11 kb)
ESM 4 (PDF 11.4 kb)


  1. 1.
    Suzuki Y, Yuen SR, Ashley R (2005) Int J Hyg Environ Health 208:201–210CrossRefGoogle Scholar
  2. 2.
    Addison J, McConnell EE (2008) Regul Toxicol Pharmacol 52:S187–S199CrossRefGoogle Scholar
  3. 3.
    Ohar J, Sterling DA, Bleecker E, Donohue J (2004) Chest 125:744–753CrossRefGoogle Scholar
  4. 4.
    Kamp DW (2009) Transl Res 153:143–152CrossRefGoogle Scholar
  5. 5.
    Kamp DW, Graceffa P, Pryor WA, Weitzman SA (1992) Free Radic Biol Med 12:293–315CrossRefGoogle Scholar
  6. 6.
    Kamp DW, Weitzman SA (1997) Proc Soc Exp Biol Med 214:12–26CrossRefGoogle Scholar
  7. 7.
    Mossman BT, Marsh JP (1989) Environ Health Perspect 81:91–94CrossRefGoogle Scholar
  8. 8.
    Drogat B, Bouchecareilh M, Petibois C, Déléris G, Chevet E, Bikfalvi A, Moenner M (2007) J Cell Physiol 212:463–472CrossRefGoogle Scholar
  9. 9.
    Petibois C, Cazorla G, Gin H, Deleris G (2001) J Lab Clin Med 137:184–190CrossRefGoogle Scholar
  10. 10.
    Petibois C, Déléris G (2004) Analyst 129:912–916CrossRefGoogle Scholar
  11. 11.
    Petibois C, Déléris G (2005) Cell Biol Int 29:709–716CrossRefGoogle Scholar
  12. 12.
    Clift MJ, Rothen-Rutishauser B, Brown DM, Duffin R, Donaldson K, Proudfoot L, Guy K, Stone V (2008) Toxicol Appl Pharmacol 232:418–427CrossRefGoogle Scholar
  13. 13.
    Petibois C, Cestelli Guidi M (2008) Anal Bioanal Chem 391:1599–1608CrossRefGoogle Scholar
  14. 14.
    Petibois C, Deleris G, Piccinini M, Cestelli Guidi M, Marcelli A (2009) Nat Photonics 3:179CrossRefGoogle Scholar
  15. 15.
    Petibois C, Desbat B (2010) Trends Biotechnol 28:495–500CrossRefGoogle Scholar
  16. 16.
    Chan J, Ying T, Guang YF, Lin LX, Kai T, Fang ZY, Ting YX, Xing LF, Ji YY (2011) Biol Trace Elem Res 144:183–196CrossRefGoogle Scholar
  17. 17.
    Carlson C, Hussain SM, Schrand AM, Braydich-Stolle LK, Hess KL, Jones RL, Schlager JJ (2008) J Phys Chem B 112:13608–13619CrossRefGoogle Scholar
  18. 18.
    Petibois C (2010) Anal Bioanal Chem 397:2051–2065CrossRefGoogle Scholar
  19. 19.
    Petibois C, Cassaigne A, Gin H, Deleris G (2004) J Clin Endocrinol Metab 89:3377–3384CrossRefGoogle Scholar
  20. 20.
    Petibois C, Piccinini M, Cestelli-Guidi M, Marcelli A (2010) J Synhrotron Rad 17:1–11CrossRefGoogle Scholar
  21. 21.
    Chan JW, Taylor DS, Zwerdling T, Lane SM, Ihara K, Huser T (2006) Biophys J 90:648–656CrossRefGoogle Scholar
  22. 22.
    Meister K, Niesel J, Schatzschneider U, Metzler-Nolte N, Schmidt DA, Havenith M (2010) Angew Chem Int Ed 49:3310–3312CrossRefGoogle Scholar
  23. 23.
    Meister K, Schmidt DA, Brundermann E, Havenith M (2010) Analyst 135:1370–1374CrossRefGoogle Scholar
  24. 24.
    Yao S, Dellaventura G, Petibois C (2010) Anal Bioanal Chem 397:2079–2089CrossRefGoogle Scholar
  25. 25.
    Taleb A, Diamond J, McGarvey JJ, Beattie JR, Toland C, Hamilton PW (2006) J Phys Chem B 110:19625–19631CrossRefGoogle Scholar
  26. 26.
    Short KW, Carpenter S, Freyer JP, Mourant JR (2005) Biophys J 88:4274–4288CrossRefGoogle Scholar
  27. 27.
    Hartmann K, Becker-Putsche M, Bocklitz T, Pachmann K, Niendorf A, Rosch P, Popp J (2012) Anal Bioanal Chem 403:745–753CrossRefGoogle Scholar
  28. 28.
    Shetty G, Kendall C, Shepherd N, Stone N, Barr H (2006) Br J Cancer 94:1460–1464CrossRefGoogle Scholar
  29. 29.
    Kammer M, Hedrich R, Ehrlich H, Popp J, Brunner E, Krafft C (2010) Anal Bioanal Chem 398:509–517CrossRefGoogle Scholar
  30. 30.
    Draux F, Gobinet C, Sule-Suso J, Trussardi A, Manfait M, Jeannesson P, Sockalingum GD (2010) Anal Bioanal Chem 397:2727–2737CrossRefGoogle Scholar
  31. 31.
    Gregas MK, Yan F, Scaffidi J, Wang HN, Vo-Dinh T (2011) Nanomedicine 7:115–122CrossRefGoogle Scholar
  32. 32.
    Kneipp K, Haka AS, Kneipp H, Badizadegan K, Yoshizawa N, Boone C, Shafer-Peltier KE, Motz JT, Dasari RR, Feld MS (2002) Appl Spectrosc 56:150–154CrossRefGoogle Scholar
  33. 33.
    Feng S, Lin J, Cheng M, Li YZ, Chen G, Huang Z, Yu Y, Chen R, Zeng H (2009) Appl Spectrosc 63:1089–1094CrossRefGoogle Scholar
  34. 34.
    Segawa H, Okuno M, Kano H, Leproux P, Couderc V, Hamaguchi HO (2012) Opt Express 20:9551–9557CrossRefGoogle Scholar
  35. 35.
    Wachsmann-Hogiu S, Weeks T, Huser T (2009) Curr Opin Biotechnol 20:63–73CrossRefGoogle Scholar
  36. 36.
    Draux F, Gobinet C, Sule-Suso J, Manfait M, Jeannesson P, Sockalingum GD (2011) Analyst 136:2718–2725CrossRefGoogle Scholar
  37. 37.
    Loo DT, Fuquay JI, Rawson CL, Barnes DW (1987) Science 236:200–202CrossRefGoogle Scholar
  38. 38.
    Leake BE, Woolley AR, Birch WD, Burke EAJ, Ferraris G, Grice JD, Hawthorne FC, Kisch HJ, Krivovichev VG, Schumacher JC, Stephenson NCN, Whittaker EJW (2004) Mineralogical Magazine 68:209–215CrossRefGoogle Scholar
  39. 39.
    Hawthorne FC, Oberti R (2007) Rev Mineralogy Geochem 67:55–88CrossRefGoogle Scholar
  40. 40.
    Mosmann T (1983) J Immunol Methods 65:55–63CrossRefGoogle Scholar
  41. 41.
    Bard D, Yarwood J, Tylee B (1997) J Raman Spectrosc 28:803–809CrossRefGoogle Scholar
  42. 42.
    Petibois C, Cestelli-Guidi M, Piccinini M, Moenner M, Marcelli A (2010) Anal Bioanal Chem 397:2123–2129CrossRefGoogle Scholar
  43. 43.
    Thebaud-Mony A (2003) Int J Occup Environ Health 9:280–286Google Scholar
  44. 44.
    Williams T, Slade P, Raeburn J (1998) Thorax 53:535CrossRefGoogle Scholar
  45. 45.
    Siskind FB (1987) Risk Anal 7:59–69CrossRefGoogle Scholar
  46. 46.
    Vanotto A (2010) Epidemiol Prev 34:15–16Google Scholar
  47. 47.
    Park J, Jeon WI, Lee SY, Ock KS, Seo JH, Ganbold EO, Cho K, Song NW, Joo SW (2012) J Biomed Mater Res A 100:1221–1228Google Scholar
  48. 48.
    Bogliolo L, Ledda S, Innocenzi P, Ariu F, Bebbere D, Rosati I, Leoni GG, Piccinini M (2012) Cryobiology 64:267–272CrossRefGoogle Scholar
  49. 49.
    Pudlas M, Koch S, Bolwien C, Thude S, Jenne N, Hirth T, Walles H, Schenke-Layland K (2011) Tissue Eng Part C Methods 17:1027–1040CrossRefGoogle Scholar
  50. 50.
    Giantomassi F, Gualtieri AF, Santarelli L, Tomasetti M, Lusvardi G, Lucarini G, Governa M, Pugnaloni A (2010) Toxicol In Vitro 24:1521–1531CrossRefGoogle Scholar
  51. 51.
    Pugnaloni A, Giantomassi F, Lucarini G, Capella S, Belmonte MM, Orciani M, Belluso E (2010) Acta Histochem 112:133–146CrossRefGoogle Scholar
  52. 52.
    Belbachir K, Lecomte S, Ta HP, Petibois C, Desbat B (2011) Anal Bioanal Chem 401:3263–3268CrossRefGoogle Scholar
  53. 53.
    Belbachir K, Noreen R, Gouspillou G, Petibois C (2009) Anal Bioanal Chem 385:829–837CrossRefGoogle Scholar
  54. 54.
    Noreen R, Moenner M, Hwu Y, Petibois C (2012) Biotechnol Adv 30:1432–1446CrossRefGoogle Scholar
  55. 55.
    Petibois C, Rigalleau V, Melin AM, Perromat A, Cazorla G, Gin H, Deleris G (1999) Clin Chem 45:1530–1535Google Scholar
  56. 56.
    Petibois C, Déléris G (2006) Trends Biotechnol 24:455–462CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Yao Seydou
    • 1
  • Hsiang-Hsin Chen
    • 1
  • Etienne Harte
    • 1
  • Giancarlo Della Ventura
    • 2
  • Cyril Petibois
    • 1
    Email author
  1. 1.Université de Bordeaux, Inserm U1029Talence-CedexFrance
  2. 2.Dipartimento Scienze GeologicheUniversità Roma TreRomeItaly

Personalised recommendations