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Analytical and Bioanalytical Chemistry

, Volume 405, Issue 21, pp 6629–6638 | Cite as

Lipid profiling of human plasma from peritoneal dialysis patients using an improved 2D (NP/RP) LC-QToF MS method

  • Min Li
  • Baosheng Feng
  • Yuan Liang
  • Wei Zhang
  • Yu Bai
  • Wen Tang
  • Tao Wang
  • Huwei LiuEmail author
Research Paper

Abstract

An improved online two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (2D LC-QToF MS) system was developed for the lipid profiling of human plasma, in which different lipid classes were separated by the first dimensional normal-phase (NP) LC and different lipid molecular species were separated by the second dimensional reversed-phase (RP) LC. This 2D LC-QToF MS system was built based on a ten-port, two-position valve as the interface, the conditions of which had been optimized and discussed in detail. As two loops were used to trap and transfer the first dimensional elute to the second dimension separately, this new interface suppressed the sample band broadening in the first dimensional column, increased the recovery and repeatability of 2D LC interface, and offered the possibility for the realization of not-stop-flow NP/RP 2D LC system. Finally, 190 endogenous lipid species out of 10 lipid classes were determined within a single run from the plasma of peritoneal patients. This method was also applied to identify the difference in lipid profile between plasma from peritoneal dialysis patients with bad volume status and peritoneal dialysis patients with good volume status. The discovery of 30 potential biomarkers would be helpful to the malnutrition, inflammation, and atherosclerosis syndrome investigation.

Keywords

Lipidomics Free fatty acids Phospholipids Two-dimensional liquid chromatography–mass spectrometry (2D LC-MS) Potential biomarkers MIA syndrome 

Abbreviations

2D LC

Two-dimensional liquid chromatography

EICs

Extracted ion chromatograms

FFA

Free fatty acids

LPC

Lysoglycerophosphocholine

LPE

Lysoglycerophosphoethanolamine

LPI

Lysoglycerophosphoinositols

LPS

Lysoglycerophosphoserine

MIA

Malnutrition, inflammation and atherosclerosis syndrome

MS

Mass spectrometry

NPLC

Normal-phase liquid chromatography

PC

Glycerophosphocholine

PCA

Principal component analysis

PE

Glycerophosphoethanolamine

PI

Glycerophosphoinositol

PS

Glycerophosphoserine

QToF

Quadrupole time-of-flight mass spectrometry

RPLC

Reversed-phase liquid chromatography

SM

Sphingomyelin

Notes

Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (grant no. 21175005). The authors wish to thank Mr. Chengsen Zhang for his kind help in drawing the figure of 2D LC interface.

Supplementary material

216_2013_7109_MOESM1_ESM.pdf (104 kb)
ESM 1 (PDF 104 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Min Li
    • 1
  • Baosheng Feng
    • 1
  • Yuan Liang
    • 1
  • Wei Zhang
    • 1
  • Yu Bai
    • 1
  • Wen Tang
    • 2
  • Tao Wang
    • 2
  • Huwei Liu
    • 1
    Email author
  1. 1.Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular EngineeringPeking UniversityBeijingChina
  2. 2.Division of Nephrology, Peking University Third HospitalBeijingChina

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