Analytical and Bioanalytical Chemistry

, Volume 405, Issue 20, pp 6599–6603 | Cite as

Capillary liquid chromatographic fingerprint used for discrimination of Zingiber montanum from related species

  • Mohamad Rafi
  • Lee Wah Lim
  • Toyohide Takeuchi
  • Latifah Kosim Darusman


Fingerprint analysis using capillary liquid chromatography (CLC) has been developed for discrimination of Zingiber montanum (ZM) from related species, for example Z. americans (ZA) and Z. zerumbet (ZZ). By comparing the fingerprint chromatograms of ZM, ZA, and ZZ we could identify ZM samples and discriminate them from ZA and ZZ by using their marker peaks. We also combined CLC fingerprint with multivariate analysis, including principal-component analysis (PCA) and canonical variate analysis (CVA); all three species were discriminated successfully. This result indicates that CLC fingerprint analysis in combination with PCA and CVA can be used for discrimination of ZM samples from samples of related species.


Zingiber montanum Zingiber americans Zingiber zerumbet Capillary liquid chromatography Fingerprint analysis Multivariate analysis 



Mohamad Rafi gratefully acknowledges the Ministry of Education and Culture of the Republic of Indonesia for the PhD scholarship in Gifu University, Japan.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Mohamad Rafi
    • 1
    • 2
  • Lee Wah Lim
    • 1
  • Toyohide Takeuchi
    • 1
  • Latifah Kosim Darusman
    • 2
    • 3
  1. 1.Department of Chemistry, Faculty of EngineeringGifu UniversityGifuJapan
  2. 2.Department of Chemistry, Faculty of Mathematics and Natural SciencesBogor Agricultural UniversityBogorIndonesia
  3. 3.Biopharmaca Research Center-Research and Community Empowerment InstituteBogor Agricultural UniversityBogorIndonesia

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