Molecularly imprinted polymers as synthetic receptors for the QCM-D-based detection of l-nicotine in diluted saliva and urine samples
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Molecularly imprinted polymers (MIPs) are synthetic receptors that are able to specifically bind their target molecules in complex samples, making them a versatile tool in biosensor technology. The combination of MIPs as a recognition element with quartz crystal microbalances (QCM-D with dissipation monitoring) gives a straightforward and sensitive device, which can simultaneously measure frequency and dissipation changes. In this work, bulk-polymerized l-nicotine MIPs were used to test the feasibility of l-nicotine detection in saliva and urine samples. First, l-nicotine-spiked saliva and urine were measured after dilution in demineralized water and 0.1× phosphate-buffered saline solution for proof-of-concept purposes. l-nicotine could indeed be detected specifically in the biologically relevant micromolar concentration range. After successfully testing on spiked samples, saliva was analyzed, which was collected during chewing of either nicotine tablets with different concentrations or of smokeless tobacco. The MIPs in combination with QCM-D were able to distinguish clearly between these samples: This proves the functioning of the concept with saliva, which mediates the oral uptake of nicotine as an alternative to the consumption of cigarettes.
KeywordsMolecularly imprinted polymers l-nicotine Nicotine tablets Smokeless tobacco Quartz crystal microbalance Dissipation monitoring
This work is supported by an IMEC Ph.D. Fellowship (J. Alenus), by the Life-Science Initiative of the Province of Limburg (M. Peeters), and by the Internationalization Program of Universidade de São Paulo, Brazil (P. Csipai). The authors also would like to thank H. Penxten, J. Soogen, C. Willems, and J. Baccus cordially for technical assistance.
- 13.Bereczki A, Tolokán A, Horvai G, Horváth V, Lanza F, Hall AJ et al (2001) Determination of phenytoin in plasma by molecularly imprinted solid-phase extraction. J Chromatogr A 1–2:31–38Google Scholar
- 21.Shi H, Tsai W-B, Garrison MD, Ferrari S, Ratner BD (1999) Template-imprinted nanostructured surfaces for protein recognition. Nature 6728:593–597Google Scholar
- 30.Neal LB (1997) The role of nicotine in smoking-related cardiovascular disease. Prev Med 4:412–417Google Scholar
- 31.Stead LF, Perera R, Mant D, Lancaster T (2008) Nicotine replacement therapy for smoking cessation (review). Cochrane Database of Systematic Reviews 1: article number CD000146Google Scholar
- 34.Schrek R, Baker LA, Ballard GP, Dolgoff S (1950) Tobacco smoking as an etiologic factor in disease. I. Cancer. Cancer Res 1:49–58Google Scholar
- 35.Doll R, Peto R (1981) The causes of cancer: quantitative estimates of avoidable risks of cancer in the United States today. J Natl Cancer Inst 6:1191Google Scholar
- 40.Robson N, Bond AJ, Wolff K (2012) Salivary nicotine and cotinine concentrations in unstimulated and stimulated saliva. SSRN eLibrary. Afr J Pharm Pharacol 4(2):061–065. Available from: http://papers.ssrn.com/sol3/papers.cfm?abstract_id=2024696. Accessed Feb 2010
- 44.Behera D, Uppal R, Majumdar S (2003) Urinary levels of nicotine & cotinine in tobacco users. Indian J Med Res 118:129–133Google Scholar
- 46.Peeters M, Csipai P, Geerets B, Weustenraed A, van Grinsven B, Gruber J, et al (2013) Heat-transfer based detection of l-nicotine, histamine, and serotonin using molecularly imprinted polymers as biomimetic receptors. Analytical and Bioanalytical Chemistry. doi: 10.1007/s00216-013-7024-9