Analytical and Bioanalytical Chemistry

, Volume 405, Issue 15, pp 5267–5278

Mass spectrometry based phospholipidomics of mammalian thymus and leukemia patients: implication for function of iNKT cells

  • Xiukun Xu
  • Yunhui Yu
  • Zheng Wang
  • Tingting Zhu
  • Yanping Wang
  • Jian Zhu
  • Zijun Chen
  • Yun He
  • Linling Ju
  • Yunsen Li
Research Paper

DOI: 10.1007/s00216-013-6923-0

Cite this article as:
Xu, X., Yu, Y., Wang, Z. et al. Anal Bioanal Chem (2013) 405: 5267. doi:10.1007/s00216-013-6923-0
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Abstract

In previous studies phospholipids have been proved to be involved in biochemical, physiological, and pathological processes. As a special class of phospholipids, peroxisome-derived lipids (PDLs) have been proved to be potential ligands of invariant natural killer T (iNKT) cells in recent studies. Here, on the basis of phospholipidomics, we focused on the relative quantity of PDLs extracted from mammalian thymus or bone marrow using electrospray ionization mass spectrometry (MS). In phospholipid analysis, we identified 12 classes of phospholipids and accounted for their relative quantities by comparing their relative abundances in the MS1 map. Our results show that PDLs are present in mammalian thymus as well as mouse spleen and liver. Interestingly, the relative quantity of PDLs extracted from human acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) bone marrows is higher than that extracted from bone marrow of healthy donors. Our results may help to explain the close correlation between PDLs and iNKT cell function in thymus, spleen, liver, and especially in leukemia patients. We think that our phospholipidomics work may reveal a function of iNKT cells.

Keywords

Phospholipids Natural killer T cells Lipidomics Peroxisome-derived lipids Function 

Abbreviations

α-GalCer

α-Galactosylceramide

AML

Acute myeloid leukemia

ALL

Acute lymphoblastic leukemia

CV

Coefficient of variation

ESI

Electrospray ionization

HPLC

High-performance liquid chromatography

iGb3

Isoglobotrihexosylceramide

iNKT

Invariant natural killer T

LC

Liquid chromatography

LPA

Lysophosphatidic acid

LPI

Lysophosphatidylinositol

MS

Mass spectrometry

NKT

Natural killer T

PDL

Peroxisome-derived lipid

PE

Phosphatidylethanolamine

PG

Phosphatidylglycerol

PS

Phosphatidylserine

Supplementary material

216_2013_6923_MOESM1_ESM.pdf (292 kb)
ESM 1(PDF 292 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Xiukun Xu
    • 1
  • Yunhui Yu
    • 1
  • Zheng Wang
    • 1
  • Tingting Zhu
    • 1
  • Yanping Wang
    • 1
  • Jian Zhu
    • 1
  • Zijun Chen
    • 2
  • Yun He
    • 1
  • Linling Ju
    • 1
  • Yunsen Li
    • 1
  1. 1.Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Jiangsu Laboratory of Infection ImmunitySoochow UniversitySuzhouChina
  2. 2.College of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghaiChina

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