Analytical and Bioanalytical Chemistry

, Volume 405, Issue 14, pp 4989–4991 | Cite as

Sensitive detection of trace hemoglobin using fluorescence method based on functionalized quantum dots

  • Houjuan Zhu
  • Suhua Wang


The fluorescence quenching of quantum dots by hemoglobin has been demonstrated to depend on surface functionalization, and this property has been utilized to construct a novel fluorescent method for rapid, sensitive, and selective detection of trace hemoglobin in urine at microgram level. This method shows low interference and high selectivity for hemoglobin with a limit of detection of 4.3 μg L−1 in water and 66.1 μg L−1 in urine, which are lower than those of currently used methods in labs and clinics. Spike and recovery tests in raw, acidified, and alkalized urine samples exhibit good recovery rates for the spiked concentrations close to the limit of detection.


Fluorescence spectra and photographs of MPA-QD solution before and after the addition of Hb taken under 365-nm irradiation.


Optical sensors Fluorescence/luminescence Nanoparticles/nanotechnology Semiconductor materials Biosensors 



This work was supported by the Natural Science Foundation of China (nos. 21077108, 21173229) and the Innovation Project of the Chinese Academy of Sciences (KJCX2-YW-H29).

Supplementary material

216_2013_6832_MOESM1_ESM.pdf (3.4 mb)
ESM 1 (PDF 3.35 mb)


  1. 1.
    Alivisatos P (2004) Nat Biotechnol 22:47–52CrossRefGoogle Scholar
  2. 2.
    Hsieh MS, Wu TG, Su CS, Chen WJ, Ozbek N, Tsai KY, Lin CY (2011) Clin Chim Acta 412:2150–2156CrossRefGoogle Scholar
  3. 3.
    Zhang K, Zhou HB, Mei QS, Wang SH, Guan GJ, Liu RY, Zhang J, Zhang ZP (2011) J Am Chem Soc 22:8424–8427CrossRefGoogle Scholar
  4. 4.
    Yu WW, Qu LH, Guo WZ, Peng XG (2003) Chem Mater 15:2854–2860CrossRefGoogle Scholar
  5. 5.
    Shen XC, Liou XY, Ye LP, Hong L, Wang ZY (2007) J Colloid Interface Sci 311:400–406CrossRefGoogle Scholar
  6. 6.
    Hu DH, Wu HM, Liang JG, Han HY (2008) Spectrochim Acta A Mol Biomol Spectrosc 69:830–834CrossRefGoogle Scholar
  7. 7.
    Liu WJ, Guo X, Guo R (2005) Int J Biol Macromol 37:232–238CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Department of ChemistryUniversity of Science and Technology of ChinaHefeiChina
  2. 2.Institute of Intelligent MachinesChinese Academy of SciencesHefeiChina

Personalised recommendations