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Analytical and Bioanalytical Chemistry

, Volume 405, Issue 12, pp 4269–4275 | Cite as

Synthesis and evaluation of molecularly imprinted polymeric microspheres for highly selective extraction of an anti-AIDS drug emtricitabine

  • Jia-ping Lai
  • Li Xie
  • Hui SunEmail author
  • Fang Chen
Original Paper

Abstract

The molecularly imprinted polymeric microspheres (MIPMs, 3∼5 μm), used as high-performance liquid chromatography (HPLC) and solid-phase extraction (SPE) packing materials for anti-AIDS drug emtricitabine (FTC), were synthesized by precipitation polymerization. The effects of ratio of chloroform to acetonitrile on the morphology and diameter of MIPMs were investigated. The prepared MIPMs were characterized by HPLC. The imprinting factor (2.26) suggests that the resultant MIPMs exhibit good recognition and affinity to FTC. In addition, the MIPMs were used in SPE as packing material for separation and enrichment of FTC. The recovery of FTC on MIPMs cartridge was 97.6 % in standard solution. Finally, the MIPMs cartridge was applied to extract the FTC in human serum samples. Impurities in sample have been mostly removed, and the average recovery of 92.5 % was obtained with a detection limit of 0.005 μg/mL and a linear range of 0.02∼4.0 μg/mL. The method established can be used to monitor the FTC in human serum sample with good accuracy and selectivity.

Keywords

Emtricitabine Molecularly imprinted polymeric microspheres (MIPMs) Anti-AIDS drugs Solid-phase extraction (SPE) Serum 

Notes

Acknowledgment

This study is financially supported by the National Nature Science Foundation of China (No: 21107018).

Supplementary material

216_2013_6818_MOESM1_ESM.pdf (218 kb)
ESM 1 (PDF 217 kb)

References

  1. 1.
    Rockstroh JK, Lennox JL, DeJesus E, Saag MS, Lazzarin A, Wan H, Walker ML, Xu X, Zhao J, Teppler H, DiNubile MJ, Rodgers AJ, Nguyen BY, Leavitt R, Sklar P (2011) Clin Infect Dis 53:807–816CrossRefGoogle Scholar
  2. 2.
    Venhoff N, Setzer B, MelkaoUi K, Walker UA (2007) Antivir Ther 12:1075–1085Google Scholar
  3. 3.
    DeJesus E, Ruane P, McDonald C, Garcia F, Sharma S, Corales R, Ravishankar J, Khanlou H, Shamblaw D, Ecker J, Ebrahimi R, Flaherty J (2008) HIV Clin Tri 9:103–114CrossRefGoogle Scholar
  4. 4.
    Pendela M, Mamade DA, Hoogmartens J, Van Schepdael A, Adams E (2010) Talanta 82:125–128CrossRefGoogle Scholar
  5. 5.
    Jansen RS, Rosing H, Kromdijk W, ter Heine R, Schellens JHM, Beijnen JH (2010) J Chromatogr B 878:621–627CrossRefGoogle Scholar
  6. 6.
    Nirogi R, Bhyrapuneni G, Kandikere V, Mudigonda K, Komarneni P, Aleti R, Mukkanti K (2009) Biomed Chromatogr 23:371–381CrossRefGoogle Scholar
  7. 7.
    Gomes NA, Vaidya VV, Pudage A, Joshi SS, Parekh SA (2008) J Pharm Biomed Anal 48:918–926CrossRefGoogle Scholar
  8. 8.
    Ashenafi D, Verbeek A, Hoogmartens J, Adams E (2009) J Separ Sci 32:1823–1830CrossRefGoogle Scholar
  9. 9.
    Seshachalam U, Haribabu B, Chandrasekhar KB (2007) J Separ Sci 30:999–1004CrossRefGoogle Scholar
  10. 10.
    Lai JP, Wang J, Cai ZW (2008) J Chromatogr B 868:1–12CrossRefGoogle Scholar
  11. 11.
    Rezk NL, Crutchley RD, Kashuba ADM (2005) J Chromatogr B 822:201–208CrossRefGoogle Scholar
  12. 12.
    Lai JP, Niessner R, Knopp D (2004) Anal Chim Acta 522:137–144CrossRefGoogle Scholar
  13. 13.
    Lai JP, He XW, Chen F (2003) Anal Bioanal Chem 377:208–213CrossRefGoogle Scholar
  14. 14.
    Turiel E, Martin-Esteban A (2004) Anal Bioanal Chem 378:1876–1886CrossRefGoogle Scholar
  15. 15.
    Gu JY, Zhang H, Yuan G, Chen LR, Xu XJ (2011) J Chromatogr A 1218:8150–8155CrossRefGoogle Scholar
  16. 16.
    Wang CY, Javadi A, Ghaffari M, Gong SQ (2010) Biomater 31:4944–4951CrossRefGoogle Scholar
  17. 17.
    Yang T, Li YH, Wei S, Li Y, Deng AP (2008) Anal Bioanal Chem 391:2905–2914CrossRefGoogle Scholar
  18. 18.
    Guo L, Deng QL, Fang GZ, Gao W, Shuo W (2011) J Chromatogr A 1218:6271–6277CrossRefGoogle Scholar
  19. 19.
    Wang XH, Chen LR, Xu XJ (2011) Anal Bioanal Chem 401:1423–1432CrossRefGoogle Scholar
  20. 20.
    Qiao FX, Geng YR, He CQ, Wu YP, Pan PY (2011) J Chromatogr B 879:2891–2896CrossRefGoogle Scholar
  21. 21.
    Ambrosini S, Shinde S, De Lorenzi E, Sellergren B (2012) Analyst 137:249–254CrossRefGoogle Scholar
  22. 22.
    Ameli A, Alizadeh N (2011) Anal Chim Acta 707:62–68CrossRefGoogle Scholar
  23. 23.
    Duy SV, Lefebvre-Tournier I, Pichon V, Hugon-Chapuis F, Puy JY, Perigaud C (2009) J Chromatogr B 877:1101–1108CrossRefGoogle Scholar
  24. 24.
    Lai JP, Yang ML, Niessner R, Knopp D (2007) Anal Bioanal Chem 389:405–412CrossRefGoogle Scholar
  25. 25.
    Dai CM, Zhou XF, Zhang YL, Liu SG, Zhang J (2011) J Hazard Mater 198:175–181CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.School of Chemistry and EnvironmentSouth China Normal UniversityGuangzhouChina
  2. 2.College of Environmental Science & EngineeringGuangzhou UniversityGuangzhouChina

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