Analytical and Bioanalytical Chemistry

, Volume 405, Issue 12, pp 4295–4305

Simultaneous determination of opiates, methadone, amphetamines, cocaine, and metabolites in human placenta and umbilical cord by LC-MS/MS

  • Ana de Castro
  • Ariana Díaz
  • Beatriz Piñeiro
  • Elena Lendoiro
  • Angelines Cruz
  • Manuel López-Rivadulla
  • Marta Concheiro
Note

DOI: 10.1007/s00216-013-6784-6

Cite this article as:
de Castro, A., Díaz, A., Piñeiro, B. et al. Anal Bioanal Chem (2013) 405: 4295. doi:10.1007/s00216-013-6784-6

Abstract

LC-MS/MS methods for the quantification of morphine, morphine-3-glucuronide, morphine-6-glucuronide, codeine, 6-acetylmorphine, cocaine, benzoylecgonine, ecgonine methyl ester, hydroxybenzoylecgonine, cocaethylene, amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), methadone, and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine in human placenta and umbilical cord were developed and validated. Specimens (1 ± 0.02 g) were homogenized with the Ultra-Turrax T8 disperser and centrifuged, and the supernatant was submitted to solid-phase extraction with Oasis MCX cartridges. Chromatographic separation was performed using an Atlantis T3 analytical column (100 × 2.1 mm, 3 μm) and a gradient of 0.1 % formic acid and acetonitrile. Selectivity was verified in 10 different blank specimens. The method was linear from 1–5 to 100–500 ng/g, depending on the analyte. Limits of detection and quantification ranged from 0.5 to 2.5 ng/g and 1 to 5 ng/g, respectively. Method imprecision was ≤15.3 %, except for MDMA at low quality control (18.1 %); accuracy, 87.1 to 114 %; extraction efficiency, 16.3 to 154.0 % (%CV = 1.8-39.4 %); matrix effect, −75.7 to 449.9 % (%CV = 3.5–50 %); and process efficiency, 8.7 to 316.0 %. The method was applied to authentic placenta and umbilical cord specimens from drug-user pregnant women.

Keywords

Placenta Umbilical cord Cocaine Methadone Opioids Amphetamines 

Supplementary material

216_2013_6784_MOESM1_ESM.pdf (192 kb)
ESM 1(PDF 192 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ana de Castro
    • 1
    • 2
  • Ariana Díaz
    • 1
  • Beatriz Piñeiro
    • 1
  • Elena Lendoiro
    • 1
  • Angelines Cruz
    • 1
  • Manuel López-Rivadulla
    • 1
  • Marta Concheiro
    • 1
    • 3
  1. 1.Toxicology Service, Institute of Forensic SciencesUniversity of Santiago de CompostelaSantiago de CompostelaSpain
  2. 2.Research Department, CienytechSantiago de CompostelaSpain
  3. 3.Chemistry and Drug Metabolism SectionNational Institute on Drug Abuse, National Institutes of HealthBaltimoreUSA

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