Analytical and Bioanalytical Chemistry

, Volume 405, Issue 10, pp 3233–3242

Trace analysis of acetylcholinesterase inhibitors with antipsychotic drugs for Alzheimer’s disease by capillary electrophoresis with on column field-amplified sample injection

  • Yi-Rou Wang
  • Yuan-Han Yang
  • Chi-Yu Lu
  • Shun-Jin Lin
  • Su-Hwei Chen
Research Paper

Abstract

A simple and sensitive capillary zone electrophoresis (CZE) with UV detection (214 nm) was developed and validated for the simultaneous determination of the acetylcholinesterase inhibitors (AChEI), donepezil, and rivastigmine, with antipsychotic drugs in plasma. A sample pretreatment by liquid–liquid extraction and subsequent quantification by CZE with field-amplified sample injection (FASI) was used. The optimum separation for these analytes was achieved in <20 min at 25 °C with a fused-silica capillary column of 60.2 cm × 50 μm I.D. (effective length 50 cm) and a run buffer containing 120 mM phosphate (pH 4.0) with 0.1 % γ-cyclodextrin, 40 % methanol (MeOH), and 0.02 % polyvinyl alcohol as a dynamic coating to reduce analytes’ interaction with the capillary wall. Using phenformin as an internal standard (40.0 ng/mL), the linear ranges of the proposed method for the simultaneous determination of donepezil, rivastigmine, aripiprazole, quetiapine, risperidone, clozapine, ziprasidone, and trazodone were over the range 4.0–80.0 ng/mL, and olanzapine was over the range 1.0–20.0 ng/mL. The method was applied for concentrations monitoring of AChEIs and antipsychotic drugs in ten Alzheimer’s disease patients with behavioral and psychological symptoms of dementia after oral administration of the commercial products.

Figure

Electropherogram of tested chemicals in plasma determinations

Keywords

Determination of acetylcholinesterase inhibitors with antipsychotic drugs Liquid–liquid extraction Field-amplified sample injection Alzheimer’s disease 

Supplementary material

216_2013_6767_MOESM1_ESM.pdf (205 kb)
ESM 1(PDF 204 kb)

References

  1. 1.
    Waller DG, Renwick AG, Hillier K (eds) (2005) Medical pharmacology and therapeutics, 2nd edn. Elsevier Saunders, St. LouisGoogle Scholar
  2. 2.
    Bartus RT, Dean RL III, Beer B, Lippa AS (1982) Science 217(4558):408–414CrossRefGoogle Scholar
  3. 3.
    Liperoti R, Pedone C, Corsonello A (2008) Curr Neuropharmacol 6:117–124CrossRefGoogle Scholar
  4. 4.
    Noetzli M, Choong E, Ansermot N, Eap CB (2011) Ther Drug Monit 33:227–238Google Scholar
  5. 5.
    Akamine Y, Yasui-Furukori N, Kojima M, Inoue Y, Uno T (2010) J Sep Sci 33:3292–3298CrossRefGoogle Scholar
  6. 6.
    Musenga A, Saracino MA, Spinelli D, Rizzato E, Boncompagni G, Kenndler E, Raggi MA (2008) Anal Chim Acta 612:204–211CrossRefGoogle Scholar
  7. 7.
    Lancelin F, Djebrani K, Tabaouti K, Kraoul L, Brovedani S, Paubel P, Piketty ML (2008) J Chromatogr B 867:15–19CrossRefGoogle Scholar
  8. 8.
    Mercolini L, Colliva C, Amore M, Fanali S, Raggi MA (2008) J Pharm Biomed Anal 47:882–887CrossRefGoogle Scholar
  9. 9.
    Mandrioli R, Mercolini L, Lateana D, Boncompagni G, Raggi MA (2011) J Chromatogr B Anal Technol Biomed Life Sci 879:167–173CrossRefGoogle Scholar
  10. 10.
    Pilli NR, Inamadugu JK, Kondreddy N, Karra VK, Damaramadugu R, Rao JVLNS (2011) Biomed Chromatogr 25:943–951CrossRefGoogle Scholar
  11. 11.
    Shah HJ, Kundlik ML, Pandya A, Prajapati S, Subbaiah G, Patel CN, Patel DM, Suhagiya BN (2009) Biomed Chromatogr 23:141–151CrossRefGoogle Scholar
  12. 12.
    Patel BN, Sharma N, Sanyal M, Shrivastav PS (2008) Anal Chim Acta 629:145–157CrossRefGoogle Scholar
  13. 13.
    Song M, Xu X, Hang T, Wen A, Yang L (2009) Anal Biochem 385:270–277CrossRefGoogle Scholar
  14. 14.
    Yeh HH, Yang YH, Ko JY, Chen SH (2008) Electrophoresis 29:3649–3657CrossRefGoogle Scholar
  15. 15.
    Hsieh YH, Yang YH, Yeh HH, Lin PC, Chen SH (2009) Electrophoresis 30:644–653CrossRefGoogle Scholar
  16. 16.
    Tsai CJ, Yu YH, Chiu HJ, Loh EW, Wang JT, Chan CH, Lan TH (2011) J Chin Med Assoc 74:267–271CrossRefGoogle Scholar
  17. 17.
    Hwang PL, Wei SY, Yeh HH, Ko JY, Chang CC, Chen SH (2010) Electrophoresis 31:2778–2786CrossRefGoogle Scholar
  18. 18.
    Farina C, Kremser L, Raggi MA, Kenndler E (2008) J Pharm Biomed Anal 46:471–476CrossRefGoogle Scholar
  19. 19.
    de Jesus LI, Albuquerque NCP, Borges KB, Simões RA, Calixto LA, Furtado NAJC, de Gaitani CM, Pupo MT, de Oliveira ARM (2011) Electrophoresis 32:1–11CrossRefGoogle Scholar
  20. 20.
    Breadmore MC (2011) J Chromatogr A 1218:1347–1352CrossRefGoogle Scholar
  21. 21.
    Wei F, Fan J, Zheng MM, Feng YQ (2010) Electrophoresis 31:714–723CrossRefGoogle Scholar
  22. 22.
    Hai X, Konečnỳ J, Zeisbergerová M, Adams E, Hoogmartens J, Van Schepdael A (2008) Electrophoresis 29:3817–3824CrossRefGoogle Scholar
  23. 23.
    Kubo M, Mizooku Y, Hirao Y, Osumi T (2008) J Chromatogr B 822:294–299CrossRefGoogle Scholar
  24. 24.
    Sentellas S, Moyano E, Puignou L, Galceran MT (2003) Electrophoresis 24:3075–3082CrossRefGoogle Scholar
  25. 25.
    Song JZ, Chen J, Tian SJ, Sun ZP (1999) J Pharm Biomed Anal 21:569–576CrossRefGoogle Scholar
  26. 26.
    Quirino JP, Terabe S (2000) J Chromatogr A 902:119–135CrossRefGoogle Scholar
  27. 27.
    Kuhn R, Hoffstetter-Kuhn S (1993) Capillary electrophoresis: principles and practice. Springer, BerlinCrossRefGoogle Scholar
  28. 28.
    Pommier F, Frigola R (2003) J Chromatogr B 784:301–313CrossRefGoogle Scholar
  29. 29.
    Patel BN, Sharma N, Sanyal M, Shrivastav PS (2008) J Chromatogr B 871:44–54CrossRefGoogle Scholar
  30. 30.
    Apostolou C, Dotsikas Y, Kousoulos C, Loukas YL (2007) J Chromatogr B 848:239–244CrossRefGoogle Scholar
  31. 31.
    Barrett B, Holčapek M, Huclová J, Bořek-Dohalský V, Fejt P, Němec B, Jelínek I (2007) J Pharm Biomed Anal 44:498–505CrossRefGoogle Scholar
  32. 32.
    Čabovska B, Cox SL, Vinks AA (2007) J Chromatogr B 852:497–504CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Yi-Rou Wang
    • 1
  • Yuan-Han Yang
    • 2
  • Chi-Yu Lu
    • 3
  • Shun-Jin Lin
    • 1
  • Su-Hwei Chen
    • 1
  1. 1.School of Pharmacy, College of PharmacyKaohsiung Medical UniversityKaohsiungTaiwan
  2. 2.Department of NeurologyKaohsiung Medical University HospitalKaohsiungTaiwan
  3. 3.Department of Biochemistry, College of MedicineKaohsiung Medical UniversityKaohsiungTaiwan

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