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Analytical and Bioanalytical Chemistry

, Volume 405, Issue 11, pp 3871–3879 | Cite as

Flexible micro(bio)sensors for quantitative analysis of bioanalytes in a nanovolume of human lachrymal liquid

  • Viktor Andoralov
  • Sergey Shleev
  • Thomas Arnebrant
  • Tautgirdas RuzgasEmail author
Original Paper

Abstract

A flexible electrochemical micro(bio)sensor has been designed for determination of several biological compounds, specifically, ascorbate, dopamine, and glucose, in human lachrymal liquid (tears). The microsensor for simultaneous determination of ascorbate and dopamine concentrations was based on a gold microwire modified with the tetrathiafulvalen–7,7,8,8-tetracyanoquinodimethane complex as a catalyst. To monitor glucose concentration in tears, glucose dehydrogenase was immobilized on a gold microwire modified with carbon nanotubes and an osmium redox polymer. A capillary microcell was constructed for sampling tears. The cell had a working volume of 60–100 nL with a sampling deviation of 6.7 %. To check if the microcell was properly filled with buffer or tear sample, a control electrode was introduced into the construction. The electrode was used to measure the electrical resistance of a fully filled nanovolume cell. The mechanical flexibility is one of the most important features of the prototype and allowed direct collection of tears with minimized risk of damage to the eye.

Figure

Tear sampling and electrochemical analysis

Keywords

Flexible micro(bio)sensor Nanovolume Tears Ascorbate Dopamine Glucose 

Notes

Acknowledgments

The authors thank Lo Gorton for the kind gift of the osmium redox polymer. The work was supported financially by the Faculty of Health and Society, Malmö University, the Swedish Research Council, and the Gustaf Th. Ohlsson Foundation.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Viktor Andoralov
    • 1
  • Sergey Shleev
    • 1
  • Thomas Arnebrant
    • 1
  • Tautgirdas Ruzgas
    • 1
    Email author
  1. 1.Department of Biomedical Sciences, Faculty of Health and SocietyMalmö UniversityMalmöSweden

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