Analytical and Bioanalytical Chemistry

, Volume 405, Issue 10, pp 3117–3123

Cloud point extraction of Δ9-tetrahydrocannabinol from cannabis resin

  • S. Ameur
  • B. Haddou
  • Z. Derriche
  • J. P. Canselier
  • C. Gourdon
Original Paper

Abstract

A cloud point extraction coupled with high performance liquid chromatography (HPLC/UV) method was developed for the determination of Δ9-tetrahydrocannabinol (THC) in micellar phase. The nonionic surfactant “Dowfax 20B102” was used to extract and pre-concentrate THC from cannabis resin, prior to its determination with a HPLC–UV system (diode array detector) with isocratic elution. The parameters and variables affecting the extraction were investigated. Under optimum conditions (1 wt.% Dowfax 20B102, 1 wt.% Na2SO4, T = 318 K, t = 30 min), this method yielded a quite satisfactory recovery rate (~81 %). The limit of detection was 0.04 μg mL−1, and the relative standard deviation was less than 2 %. Compared with conventional solid–liquid extraction, this new method avoids the use of volatile organic solvents, therefore is environmentally safer.

Keywords

Cannabis Δ9-Tetrahydrocannabinol Cloud point extraction Coacervate HPLC Diode array detector Nonionic surfactant 

Abbreviations

ASE

Accelerated solvent extraction

CBD

Cannabidiol

CBN

Cannabinol

CPE

Cloud point extraction

CPT

Cloud point temperature

DAD

Photodiode array detector

HPLC

High performance liquid chromatography

LLE

Liquid–liquid extraction

LOD

Limit of detection

RSD

Relative standard deviation

SPE

Solid-phase extraction

SPME

Solid-phase microextraction

THC

Δ9-Tetrahydrocannabinol

THCA

Tetrahydrocannabinolic acid

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • S. Ameur
    • 1
  • B. Haddou
    • 1
  • Z. Derriche
    • 1
  • J. P. Canselier
    • 2
  • C. Gourdon
    • 2
  1. 1.Faculté des Sciences, Département de Chimie, Laboratoire de Physico Chimie des MatériauxU. S. T. OranOranAlgeria
  2. 2.Laboratoire de Génie ChimiqueUMR 5503, Campus INP-ENSIACETToulouseFrance

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