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Analytical and Bioanalytical Chemistry

, Volume 405, Issue 8, pp 2525–2533 | Cite as

Preparation of an amperometric sensor for norfloxacin based on molecularly imprinted grafting photopolymerization

  • Zhihua WangEmail author
  • Jinshu Li
  • Xiaole Liu
  • Jianming Yang
  • Xiaoquan LuEmail author
Original Paper

Abstract

A sensitive amperometric sensor for norfloxacin (NF) was introduced. The receptor layer was prepared by molecularly imprinted photopolymerization of acrylamide and trimethylolpropane trimethacrylate on the surface of a gold electrode. The binding mechanism of the molecularly imprinted polymer was explored by ultraviolet (UV) and infrared (IR) spectroscopy. The chemosensor was characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance (EI), and scanning electron microscopy (SEM). The electrode prepared by photopolymerization has a better recognition ability to template molecules than that of electropolymerization and NIP. Some parameters affecting sensor response were optimized. Norfloxacin was detected by measurements of an amperometric it curve. The linear relationships between current and logarithmic concentration are obtained from 1.0 × 10−9 to 1.0 × 10−3 mol L−1. The detection limit of the sensor was 1.0 × 10−10 mol L−1. The proposed method is sensitive, simple, and cheap, and is applied to detect NF in human urine successfully.

Figure

Amperometric i-t curves of MIPs electrode

Keywords

Molecularly imprinted polymer Norfloxacin Photopolymerization Sensor 

Notes

Acknowledgments

This work is supported by the National Natural Science Foundation of China (No. 20965007). We also thank the Key Laboratory of Eco-Environment-Related Polymer Materials (Northwest Normal University), Ministry of Education, for financial support.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical EngineeringNorthwest Normal UniversityLanzhouChina

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