Analytical and Bioanalytical Chemistry

, Volume 405, Issue 8, pp 2505–2514 | Cite as

Binding kinetics of human cellular prion detection by DNA aptamers immobilized on a conducting polypyrrole

  • A. Miodek
  • A. Poturnayová
  • M. Šnejdárková
  • T. Hianik
  • H. Korri-Youssoufi
Original Paper

Abstract

We developed a biosensor based on the surface plasmon resonance (SPR) method for the study of the binding kinetics and detection of human cellular prions (PrPC) using DNA aptamers as bioreceptors. The biosensor was formed by immobilization of various biotinylated DNA aptamers on a surface of conducting polypyrrole modified by streptavidin. We demonstrated that PrPC interaction with DNA aptamers could be followed by measuring the variation of the resonance angle. This was studied using DNA aptamers of various configurations, including conventional single-stranded aptamers that contained a rigid double-stranded supporting part and aptamer dimers containing two binding sites. The kinetic constants determined by the SPR method suggest strong interaction of PrPC with various DNA aptamers depending on their configuration. SPR aptasensors have a high selectivity to PrPC and were regenerable by a brief wash in 0.1 M NaOH. The best limit of detection (4 nM) has been achieved with this biosensor based on DNA aptamers with one binding site but containing a double-stranded supporting part.

Fig

Aptasensors for kinetic evaluation and detection of prions by SPR

Keywords

Prions Aptamers Kinetics SPR Polypyrrole 

Supplementary material

216_2012_6665_MOESM1_ESM.pdf (127 kb)
ESM 1(PDF 127 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • A. Miodek
    • 1
  • A. Poturnayová
    • 2
  • M. Šnejdárková
    • 2
  • T. Hianik
    • 3
  • H. Korri-Youssoufi
    • 1
  1. 1.CNRS UMR-8182, Institut de Chimie Moléculaire et des Matériaux d’OrsayEquipe de Chimie Bioorganique et Bioinorganique, Univ Paris-SudOrsayFrance
  2. 2.Institute of Animal Biochemistry and GeneticsSlovak Academy of SciencesIvanka pri DunajiSlovakia
  3. 3.Faculty of Mathematics, Physics and InformaticsComenius UniversityBratislavaSlovakia

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