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Analytical and Bioanalytical Chemistry

, Volume 405, Issue 1, pp 307–314 | Cite as

Analysis of intercellular communication by flexible hydrodynamic gating on a microfluidic chip

  • Peng Chen
  • Pu Chen
  • Xiaojun Feng
  • Wei Du
  • Bi-Feng LiuEmail author
Original Paper

Abstract

Intercellular Ca2+ waves are propagation of Ca2+ transients among cells that could be initiated by chemical stimulation. Current methods for analyzing intercellular Ca2+ waves are difficult to realize localized chemical stimulations upon the target cell without interfering with adjacent contacting cells. In this paper, a simple and flexible microfluidic method was developed for investigating the intercellular communication of Ca2+ signals. A cross-patterned microfluidic chip was designed and fabricated with polydimethylsiloxane as the structural material. Localized chemical stimulation was achieved by a new strategy based on hydrodynamic gating technique. Clusters of target cells were seeded at the location within 300 μm downstream of the intersection of the cross-shaped microchannel. Confined lateral molecular diffusion largely minimized the interference from diffusion-induced stimulation of adjacent cells. Localized stimulation of the target cell with adenosine 5′-triphosphate successfully induced the propagation of intercellular Ca2+ waves among a population of adjacent contacting cells. Further inhibition studies verified that the propagation of calcium signals among NIH-3 T3 cells was dependent on direct cytosolic transfer via gap junctions. The developed microfluidic method provides a versatile platform for investigating the dynamics of intercellular communications.

Fig

Analysis of intercellular communication by flexible hydrodynamic gating

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Keywords

Microfluidic chip Hydrodynamic gating Intercellular communication Ca2+ waves 

Notes

Acknowledgments

The authors gratefully acknowledge the financial supports from National Basic Research Program of China (2011CB910403) and National Natural Science Foundation of China (30970692 and 21075045).

Supplementary material

216_2012_6447_MOESM1_ESM.pdf (1.2 mb)
ESM 1 (PDF 1236 kb)
Video S1

Numerical simulations of different gating and injection states (MPG 3090 kb)

Video S2

Visualization study of different gating and injection states (MPG 2756 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Peng Chen
    • 1
  • Pu Chen
    • 1
  • Xiaojun Feng
    • 1
  • Wei Du
    • 1
  • Bi-Feng Liu
    • 1
    Email author
  1. 1.Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics and Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhanChina

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