Analytical and Bioanalytical Chemistry

, Volume 404, Issue 8, pp 2287–2294

Simultaneous determination of cyanide and thiocyanate in plasma by chemical ionization gas chromatography mass-spectrometry (CI-GC-MS)

  • Raj K. Bhandari
  • Robert P. Oda
  • Stephanie L. Youso
  • Ilona Petrikovics
  • Vikhyat S. Bebarta
  • Gary A. Rockwood
  • Brian A. Logue
Original Paper

DOI: 10.1007/s00216-012-6360-5

Cite this article as:
Bhandari, R.K., Oda, R.P., Youso, S.L. et al. Anal Bioanal Chem (2012) 404: 2287. doi:10.1007/s00216-012-6360-5

Abstract

An analytical method utilizing chemical ionization gas chromatography-mass spectrometry was developed for the simultaneous determination of cyanide and thiocyanate in plasma. Sample preparation for this analysis required essentially one-step by combining the reaction of cyanide and thiocyanate with pentafluorobenzyl bromide and simultaneous extraction of the product into ethyl acetate facilitated by a phase-transfer catalyst, tetrabutylammonium sulfate. The limits of detection for cyanide and thiocyanate were 1 μM and 50 nM, respectively. The linear dynamic range was from 10 μM to 20 mM for cyanide and from 500 nM to 200 μM for thiocyanate with correlation coefficients higher than 0.999 for both cyanide and thiocyanate. The precision, as measured by %RSD, was below 9 %, and the accuracy was within 15 % of the nominal concentration for all quality control standards analyzed. The gross recoveries of cyanide and thiocyanate from plasma were over 90 %. Using this method, the toxicokinetic behavior of cyanide and thiocyanate in swine plasma was assessed following cyanide exposure.

Keywords

Cyanide Thiocyanate Method development Chemical-ionization gas-chromatography mass-spectrometry 

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Raj K. Bhandari
    • 1
  • Robert P. Oda
    • 1
  • Stephanie L. Youso
    • 1
  • Ilona Petrikovics
    • 2
  • Vikhyat S. Bebarta
    • 3
  • Gary A. Rockwood
    • 4
  • Brian A. Logue
    • 1
  1. 1.Department of Chemistry and BiochemistrySouth Dakota State UniversityBrookingsUSA
  2. 2.Department of ChemistrySam Houston State UniversityHuntsvilleUSA
  3. 3.Medical Toxicology, San Antonio Military Medical CenterUnited States Air ForceSan AntonioUSA
  4. 4.Analytical Toxicology DivisionUS Army Medical Research Institute of Chemical DefenseAberdeen Proving GroundUSA

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