Analytical and Bioanalytical Chemistry

, Volume 404, Issue 3, pp 785–792 | Cite as

Potential-modulated fluorescence spectroscopy of zwitterionic and dicationic membrane-potential-sensitive dyes at the 1,2-dichloroethane/water interface

  • Toshiyuki Osakai
  • Tatsuya Yoshimura
  • Daichi Kaneko
  • Hirohisa Nagatani
  • Sang-Hyun Son
  • Yutaka Yamagishi
  • Koji Yamada
Original Paper

Abstract

The previously introduced technique of potential-modulated fluorescence (PMF) spectroscopy was used to study the potential-induced fluorescence change of some different dyes at the polarized 1,2-dichloroethane (DCE)/water (W) interface. A zwitterionic dye (POLARIC 488PPS) showed a PMF response similar to that for the previously studied dye (di-4-ANEPPS) with the same ionic state, and the PMF response was likewise explained by the potential-dependent reorientation of the dye at the DCE/W interface. Though a monocationic dye (POLARIC 488PM) showed no distinct PMF signal, a dicationic dye (di-2-ANEPEQ) showed two relatively weak but detectable PMF signals at lower and higher potential. It has thus been found that the ionic state of a potential-sensitive dye strongly influences the potential-induced reorientation of the dye at the interface and consequently its PMF response. These results support the reorientation/solvatochromic mechanism proposed for “slow” dyes but do not necessarily exclude the electrochromic mechanism proposed for “fast” dyes. PMF spectroscopy would provide useful information on the design of slow dyes for the measurement of the resting potential of cell membranes

Fig

Potential-dependent reorientation of a zwitterionic membrane-potential-sensitive dye (POLARIC 488PPS) at the oil/water interface and its potential-modulated fluorescence signal (inset)

Keywords

Membrane-potential-sensitive dye POLARIC™ Liquid/liquid interface Potential modulated fluorescence 

Notes

Acknowledgments

T. Osakai, T. Yoshimura, and D. Kaneko acknowledge Dr. Shunji Kasahara and Dr. Seiji Akimoto of Kobe University for their helpful pieces of advice in the spectroscopic measurements. We also thank Goryo Chemical, Inc. for supplying POLARIC™ probes.

Supplementary material

216_2012_6199_MOESM1_ESM.pdf (896 kb)
ESM 1 (PDF 895 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Toshiyuki Osakai
    • 1
  • Tatsuya Yoshimura
    • 1
  • Daichi Kaneko
    • 1
  • Hirohisa Nagatani
    • 2
  • Sang-Hyun Son
    • 3
  • Yutaka Yamagishi
    • 3
  • Koji Yamada
    • 3
  1. 1.Department of Chemistry, Graduate School of ScienceKobe UniversityKobeJapan
  2. 2.Faculty of Chemistry, Institute of Science and EngineeringKanazawa UniversityKanazawaJapan
  3. 3.Section of Materials Science, Faculty of Environmental Earth ScienceHokkaido UniversitySapporoJapan

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