Analytical and Bioanalytical Chemistry

, Volume 403, Issue 8, pp 2397–2402

A method to determine the kinetics of multiple proteins in human infants with respiratory distress syndrome

  • Michael S. Bereman
  • Daniela M. Tomazela
  • Hillary S. Heins
  • Manuela Simonato
  • Paola E. Cogo
  • Aaron Hamvas
  • Bruce W. Patterson
  • F. Sessions Cole
  • Michael J. MacCoss
Original Paper

Abstract

We report a method to measure in vivo turnover of four proteins from sequential tracheal aspirates obtained from human newborn infants with respiratory distress syndrome using targeted proteomics. We detected enrichment for all targeted proteins approximately 3 h from the start of infusion of [5,5,5-2H3] leucine, secretion times that varied from 1.2 to 2.5 h, and half lives that ranged between 10 and 21 h. Complement factor B, a component of the alternative pathway of complement activation, had an approximately twofold-longer half-life than the other three proteins. In addition, the kinetics of mature and carboxy-terminal tryptic peptides from the same protein (surfactant protein B) were not statistically different (p = 0.49).

Keywords

Protein turnover Respiratory distress syndrome Selected reaction monitoring SRM Protein kinetics Protein metabolism 

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Michael S. Bereman
    • 1
  • Daniela M. Tomazela
    • 1
  • Hillary S. Heins
    • 2
  • Manuela Simonato
    • 3
  • Paola E. Cogo
    • 4
  • Aaron Hamvas
    • 2
  • Bruce W. Patterson
    • 5
  • F. Sessions Cole
    • 2
  • Michael J. MacCoss
    • 1
  1. 1.Department of Genome SciencesUniversity of WashingtonSeattleUSA
  2. 2.Division of Newborn Medicine, Edward Mallinckrodt Department of PediatricsWashington University School of Medicine/St. Louis Children’s HospitalSt. LouisUSA
  3. 3.Department of PediatricsUniversity of PadovaPadovaItaly
  4. 4.Bambino Gesù Pediatric Hospital, IRCCSRomeItaly
  5. 5.Department of Medicine and Center for Human NutritionWashington University School of MedicineSt. LouisUSA

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