Analytical and Bioanalytical Chemistry

, Volume 403, Issue 3, pp 807–819 | Cite as

Low–medium resolution HLA-DQ2/DQ8 typing for coeliac disease predisposition analysis by colorimetric assay

  • Hamdi Joda
  • Valerio Beni
  • Deirdre Curnane
  • Ioanis Katakis
  • Noora Alakulppi
  • Jukka Partanen
  • Kristina Lind
  • Linda Strömbom
  • Ciara K. O’Sullivan
Original Paper

Abstract

Coeliac disease is an inflammation of the small intestine, occurring in genetically susceptible individuals triggered by the ingestion of gluten. Human Leukocyte Antigens (HLA) DQ2 and DQ8 gene have been identified as key genetic factors in coeliac disease as they are presented in almost 100 % of the patients. These genes are encoded by the combination of certain alleles in the DQA and DQB region of chromosome 6. Specifically, DQA1*05:01 and DQB1*02:01 alleles for serologically defined leukocyte antigen DQ2 cis, DQA1*05:05 and DQB1*02:02 for DQ2 trans and DQA1*03:01 and DQB1*03:02 alleles for the DQ8. Specific identification of these alleles is a challenge due to the high number of alleles that have been identified so far: 46 in the DQA region and 160 in the DQB region (as of IMGT/HLA Database 10/2011 release). In the reported work, the development of a multiplex colorimetric assay for the low to medium HLA typing of the DQ2 and DQ8 genes is presented. The optimisation of probe design and assay conditions, performed by both surface plasmon resonance and enzyme-linked oligonucleotide assay, are reported. Finally, the performances of the developed typing platform were validated by the analysis of real patient samples and HLA typing, compared with those obtained using hospital based typing technology and an excellent correlation obtained.

Figure

Example of the results obtained during the colorimetric HLA typing of a real sample

Keywords

Coeliac disease HLA typing Sequence specific oligonucleotide probes SPR ELONA 

Supplementary material

216_2012_5898_MOESM1_ESM.pdf (1.2 mb)
ESM 1(PDF 1.19 Mb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Hamdi Joda
    • 1
  • Valerio Beni
    • 1
    • 2
  • Deirdre Curnane
    • 1
  • Ioanis Katakis
    • 1
  • Noora Alakulppi
    • 3
  • Jukka Partanen
    • 3
  • Kristina Lind
    • 4
  • Linda Strömbom
    • 4
  • Ciara K. O’Sullivan
    • 1
    • 5
  1. 1.Departament d’Enginyeria QuimicaUniversitat Rovira i VirgiliTarragonaSpain
  2. 2.Department of Physics, Chemistry and Biology (IFM)Linköping UniversityLinköpingSweden
  3. 3.Finnish Red Cross Blood ServiceHelsinkiFinland
  4. 4.TATAA Biocenter ABGöteborgSweden
  5. 5.Institucio Catalana de Recerca i Estudis AvançatsBarcelonaSpain

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