Analytical and Bioanalytical Chemistry

, Volume 403, Issue 2, pp 601–611 | Cite as

Two-dimensional liquid chromatography of PDMS–PS block copolymers

  • Muhammad Imran MalikEmail author
  • Gareth W. Harding
  • Harald Pasch
Original Paper


In this study, liquid chromatography at critical conditions of polystyrene (PS) and polydimethylsiloxane (PDMS) is used as the first dimension for the two-dimensional analysis of polydimethylsiloxane-block-polystyrene copolymers. Comprehensive two-dimensional liquid chromatography with size exclusion chromatography as the second dimension reveals information about the molar mass distributions of all separated fractions from the first dimension. Furthermore, fractions eluting at the critical conditions were collected and subjected to analysis in the second dimension at the critical adsorption point of the other block. These fractions were analyzed by Fourier transform infrared spectroscopy to determine their chemical compositions. The combination of the above approaches and the calibration of the evaporative light scattering detector for the first-dimension analysis yield deep insights into the molecular heterogeneity of the block copolymer samples. The composition of the samples and the chemical composition of the real block copolymer are also calculated by combining the results obtained at both critical conditions.


Hybrid materials Block copolymers Critical adsorption point (CAP) Liquid chromatography at critical conditions (LCCC) Polydimethylsiloxane (PDMS) Polystyrene (PS) Two-dimensional liquid chromatography (2D-LC) FTIR 



MIM thanks the Claude Leon Foundation for a postdoctoral fellowship. The authors also extend their gratitude to Prof. Peter E. Mallon and Dr. Gareth M. Bayley for providing samples.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Muhammad Imran Malik
    • 1
    • 2
    Email author
  • Gareth W. Harding
    • 1
  • Harald Pasch
    • 1
  1. 1.Department of Chemistry and Polymer ScienceUniversity of StellenboschMatielandSouth Africa
  2. 2.H.E.J. Research Institute of Chemistry, International Centre for Chemical and Biological Sciences (ICCBS)University of KarachiKarachiPakistan

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