Analytical and Bioanalytical Chemistry

, Volume 402, Issue 9, pp 2923–2933

A reversed-phase capillary ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) method for comprehensive top-down/bottom-up lipid profiling

  • Xiaoli Gao
  • Qibin Zhang
  • Da Meng
  • Giorgis Isaac
  • Rui Zhao
  • Thomas L. Fillmore
  • Rosey K. Chu
  • Jianying Zhou
  • Keqi Tang
  • Zeping Hu
  • Ronald J. Moore
  • Richard D. Smith
  • Michael G. Katze
  • Thomas O. Metz
Original Paper

DOI: 10.1007/s00216-012-5773-5

Cite this article as:
Gao, X., Zhang, Q., Meng, D. et al. Anal Bioanal Chem (2012) 402: 2923. doi:10.1007/s00216-012-5773-5

Abstract

Lipidomics is a critical part of metabolomics and aims to study all the lipids within a living system. We present here the development and evaluation of a sensitive capillary UPLC-MS method for comprehensive top-down/bottom-up lipid profiling. Three different stationary phases were evaluated in terms of peak capacity, linearity, reproducibility, and limit of quantification (LOQ) using a mixture of lipid standards representative of the lipidome. The relative standard deviations of the retention times and peak abundances of the lipid standards were 0.29% and 7.7%, respectively, when using the optimized method. The linearity was acceptable at >0.99 over 3 orders of magnitude, and the LOQs were sub-fmol. To demonstrate the performance of the method in the analysis of complex samples, we analyzed lipids extracted from a human cell line, rat plasma, and a model human skin tissue, identifying 446, 444, and 370 unique lipids, respectively. Overall, the method provided either higher coverage of the lipidome, greater measurement sensitivity, or both, when compared to other approaches of global, untargeted lipid profiling based on chromatography coupled with MS.

Keywords

Ultra-performance liquid chromatography (UPLC) Tandem mass spectrometry (MS/MS) Electrospray ionization (ESI) Top-down/bottom-up lipid profiling 

Supplementary material

216_2012_5773_MOESM1_ESM.pdf (3.1 mb)
ESM 1(PDF 3.11 mb)

Copyright information

© Springer-Verlag (outside the USA) 2012

Authors and Affiliations

  • Xiaoli Gao
    • 1
  • Qibin Zhang
    • 1
  • Da Meng
    • 1
  • Giorgis Isaac
    • 1
  • Rui Zhao
    • 2
  • Thomas L. Fillmore
    • 2
  • Rosey K. Chu
    • 2
  • Jianying Zhou
    • 1
  • Keqi Tang
    • 1
  • Zeping Hu
    • 1
  • Ronald J. Moore
    • 1
  • Richard D. Smith
    • 1
  • Michael G. Katze
    • 3
    • 4
  • Thomas O. Metz
    • 1
  1. 1.Biological Sciences DivisionPacific Northwest National LaboratoryRichlandUSA
  2. 2.Environmental Molecular Sciences LaboratoryPacific Northwest National LaboratoryRichlandUSA
  3. 3.Department of Microbiology, School of MedicineUniversity of WashingtonSeattleUSA
  4. 4.Washington National Primate Research CenterUniversity of WashingtonSeattleUSA

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