A photosynthetic biosensor with enhanced electron transfer generation realized by laser printing technology
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One of the limits of current electrochemical biosensors is a lack of methods providing stable and highly efficient junctions between biomaterial and solid-state devices. This paper shows how laser-induced forward transfer (LIFT) can enable efficient electron transfer from photosynthetic biomaterial immobilized on screen-printed electrodes (SPE). The ideal pattern, in terms of photocurrent signal of thylakoid droplets giving a stable response signal with a current intensity of approximately 335 ± 13 nA for a thylakoid mass of 28 ± 4 ng, was selected. It is shown that the efficiency of energy production of a photosynthetic system can be strongly enhanced by the LIFT process, as demonstrated by use of the technique to construct an efficient and sensitive photosynthesis-based biosensor for detecting herbicides at nanomolar concentrations.
KeywordsLaser printing Biosensor Photosynthesis Herbicides
The work discussed in this paper used technology developed in projects with financial support from the European Commission (e-LIFT FP7 ICT, grant agreement no. 247868; BEEP-C-EN FP 7-SME-2008-01, grant agreement no. 231082, SENSBIOSYN FP 7-SME-2008-01, grant agreement no. 232522), which is gratefully acknowledged.
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