Analytical and Bioanalytical Chemistry

, Volume 402, Issue 6, pp 2217–2220 | Cite as

Highly sensitive detection of microRNA by chemiluminescence based on enzymatic polymerization

  • Changbei Ma
  • Edward S. YeungEmail author
  • Shengda Qi
  • Rui Han
Technical Note


We have developed a new methodology for miRNA assay using chemiluminescence imaging by poly(U) polymerase catalyzed miRNA polymerization. This method is very sensitive with a 50 fM limit of detection, which is comparable to or better than current assay methods. Multiplex detection for miRNA can be easily realized by introducing different capture probes onto the biosensor array, which will make it highly versatile for various research purposes.


MicroRNA Chemiluminescence Array 



The Ames Laboratory is operated for the U.S. Department of Energy by Iowa State University under Contract No. DE-AC02-07CH11358. This work was supported by the Director of Science, Office of Basic Energy Science, Division of Chemical Sciences.


  1. 1.
    Bartel DP (2004) Cell 116:281–297CrossRefGoogle Scholar
  2. 2.
    Ambros V (2004) Nature 431:350–355CrossRefGoogle Scholar
  3. 3.
    Tavazoie SF, Alarcon C, Oskarsson T, Padua D, Wang Q, Bos PD, Gerald WL, Massague J (2008) Nature 451:147–152CrossRefGoogle Scholar
  4. 4.
    Wienholds E, Kloosterman WP, Miska E, Alvarez-Saave-dra E, Berezikov E, de Bruijn E, Horvitz HR, Kauppinen S, Plasterk RHA (2005) Science 309:310–311CrossRefGoogle Scholar
  5. 5.
    Bartels CL, Tsongalis GJ (2009) Clin Chem 55:623–631CrossRefGoogle Scholar
  6. 6.
    Calin GA, Croce CM (2006) Nat Rev Cancer 6:857–866CrossRefGoogle Scholar
  7. 7.
    Hebert SS, De Strooper B (2007) Science 317:1179–1180CrossRefGoogle Scholar
  8. 8.
    Walker MD (2008) Diabetes 57:2567–2568CrossRefGoogle Scholar
  9. 9.
    Cullen BR (2009) Nature 457:421–425CrossRefGoogle Scholar
  10. 10.
    Croce CM, Calin GA (2005) Cell 122:6–7CrossRefGoogle Scholar
  11. 11.
    Wark AW, Lee HJ, Corn RM (2008) Angew Chem Int Ed 47:644–652CrossRefGoogle Scholar
  12. 12.
    Cissell KA, Shrestha S, Deo SK (2007) Anal Chem 79:4754–4761CrossRefGoogle Scholar
  13. 13.
    Valoczi A, Hornyik C, Varga N, Burgyan J, Kauppinen S, Havelda Z (2004) Nucleic Acids Res 32:e175CrossRefGoogle Scholar
  14. 14.
    Nelson PT, Baldwin DA, Scearce LM, Oberholtzer JC, Tobias JW, Mourelatos Z (2004) Nat Methods 1:155–161CrossRefGoogle Scholar
  15. 15.
    Kloosterman WP, Wienholds E, de Bruijn E, Kauppinen S, Plasterk RH (2006) Nat Methods 3:27–29CrossRefGoogle Scholar
  16. 16.
    Lusi EA, Passamano M, Guarascio P, Scarpa A, Schiavo L (2009) Anal Chem 81:2819–2822CrossRefGoogle Scholar
  17. 17.
    Pohlmann C, Sprinzl M (2010) Anal Chem 82:4434–4440CrossRefGoogle Scholar
  18. 18.
    Fan Y, Chen X, Trigg AD, Tung C, Kong J, Gao ZJ (2007) Am Chem Soc 129:5437–5443CrossRefGoogle Scholar
  19. 19.
    Yang H, Hui A, Pampalakis G, Soleymani L, Liu FF, Sargent EH, Kelley SO (2009) Angew Chem Int Ed 48:8461–8464CrossRefGoogle Scholar
  20. 20.
    Wanunu M, Dadosh T, Ray V, Jin J, McReynolds L, Drndic M (2010) Nat Nanotech 5:807–814CrossRefGoogle Scholar
  21. 21.
    Liang RQ, Li W, Li Y, Tan CY, Li JX, Jin YX, Ruan KC (2005) Nucleic Acids Res 33:e17CrossRefGoogle Scholar
  22. 22.
    Cheng Y, Zhang X, Li Z, Jiao X, Wang Y, Zhang Y (2009) Angew Chem Int Ed 48:3268–3272CrossRefGoogle Scholar
  23. 23.
    Jia H, Li Z, Liu C, Cheng Y (2010) Angew Chem Int Ed 49:5498–5501CrossRefGoogle Scholar
  24. 24.
    Chan HM, Chan LS, Wong RN, Li HW (2010) Anal Chem 82:6911–6918CrossRefGoogle Scholar
  25. 25.
    Chang PL, Chang YS, Chen JH, Chen SJ, Chen HC (2008) Anal Chem 80:8554–8560CrossRefGoogle Scholar
  26. 26.
    Li N, Jablonowski C, Jin H, Zhong W (2009) Anal Chem 81:4906–4913CrossRefGoogle Scholar
  27. 27.
    Fang S, Lee HJ, Wark AW, Corn RMJ (2006) Am Chem Soc 128:14044–14046CrossRefGoogle Scholar
  28. 28.
    Sipova H, Zhang S, Dudley AM, Galas D, Wang K, Homola J (2010) Anal Chem 82:10110–10115CrossRefGoogle Scholar
  29. 29.
    Husale S, Persson HHJ, Sahin O (2009) Nature 462:1075–1078CrossRefGoogle Scholar
  30. 30.
    Zhang Y, Phillips GJ, Li QX, Yeung ES (2008) Anal Chem 80:9316–9325CrossRefGoogle Scholar
  31. 31.
    Zhang Y, Phillips GJ, Yeung ES (2007) Anal Chem 79:5373–5381CrossRefGoogle Scholar
  32. 32.
    Zhang Y, Phillips GJ, Yeung ES (2008) Anal Chem 80:597–605CrossRefGoogle Scholar
  33. 33.
    Kang SH, Lee S, Yeung ES (2010) Angew Chem Int Ed 49:2603–2606CrossRefGoogle Scholar
  34. 34.
    Castoldi M, Schmidt S, Benes V, Noerholm M, Kulozik AE, Hentze MW, Muckenthaler MU (2006) RNA 12:913–920CrossRefGoogle Scholar
  35. 35.
    Lee JM, Cho H, Jung Y (2010) Angew Chem Int Ed 49:8662–8665CrossRefGoogle Scholar
  36. 36.
    Neely LA, Patel S, Garver J, Gallo M, Hackett M, McLaughlin S, Nadel M, Harris J, Gullans S, Rooke J (2006) Nat Methods 3:41–46CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Changbei Ma
    • 1
  • Edward S. Yeung
    • 1
    Email author
  • Shengda Qi
    • 1
  • Rui Han
    • 1
  1. 1.Ames Laboratory-USDOE and Department of ChemistryIowa State UniversityAmesUSA

Personalised recommendations