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Analytical and Bioanalytical Chemistry

, Volume 402, Issue 10, pp 3245–3252 | Cite as

Optical sensors with molecularly imprinted nanospheres: a promising approach for robust and label-free detection of small molecules

  • Felix KolarovEmail author
  • Klaus Niedergall
  • Monika Bach
  • Günter E. M. Tovar
  • Günter Gauglitz
Original Paper

Abstract

Molecularly imprinted nanospheres obtained by miniemulsion polymerization have been applied as the sensitive layer for label-free direct optical sensing of small molecules. Using these particles as the sensitive layer allowed for improving response times in comparison to sensors using MIP layers. As a model compound, well-characterized nanospheres imprinted against l-Boc-phenylalanine anilide (l-BFA) were chosen. For immobilization, a simple concept based on electrostatic adsorption was used, showing its applicability to different types of surfaces, leading to a good surface coverage. The sensor showed short response times, good selectivity, and high reversibility with a limit of detection down to 60 μM and a limit of quantitation of 94 μM. Furthermore, reproducibility, selectivity, and long-term stability of the sensitive layers were tested. The best results were achieved with an adsorption on aminopropylsilane layers, showing a chip-to-chip reproducibility of 22%. Furthermore, the sensors showed no loss in signal after a storage time of 1 year.

Keywords

Molecularly imprinted polymer RIfS Label free Optical sensors Miniemulsion polymerization l-BFA 

Notes

Acknowledgment

The work presented in this paper was funded by the “Biomimetischer Prozesssensor” research project funded by the Ministry of Science and Culture (Ministerium für Wissenschaft und Kultur), Baden Württemberg.

Supplementary material

216_2011_5592_MOESM1_ESM.pdf (32 kb)
ESM 1 (PDF 31.6 kb)

References

  1. 1.
    Fechner P, Pröll F, Albrecht C, Gauglitz G (2011) Anal Bioanal Chem 400:729–735CrossRefGoogle Scholar
  2. 2.
    Ruigrok VJB, Levisson M, Eppink MHM, Smidt H, van der Oost J (2011) Biochem J 436:1–13CrossRefGoogle Scholar
  3. 3.
    Albrecht C, Fechner P, Honcharenko D, Baltzer L, Gauglitz G (2010) Biosens Bioelectron 25:2302–2308CrossRefGoogle Scholar
  4. 4.
    Wulff G (1995) Angew Chem Int Edn 34:1812–1832CrossRefGoogle Scholar
  5. 5.
    Mosbach K (1994) Trends Biochem Sci 19:9–14CrossRefGoogle Scholar
  6. 6.
    Delaney TL, Zimin D, Rahm M, Weiss D, Wolfbeis OS, Mirsky VM (2007) Anal Chem 79:3220–3225CrossRefGoogle Scholar
  7. 7.
    Brüggemann O, Haupt K, Ye L, Yilmaz E, Mosbach K (2000) J Chrom A 889:15–24CrossRefGoogle Scholar
  8. 8.
    Vaihinger D, Landfester K, Kräuter I, Brunner H, Tovar GEM (2002) Macromol Chem Phys 203:1965–1973CrossRefGoogle Scholar
  9. 9.
    Henry OYF, Cullen DC, Piletsky SA (2005) Anal Bioanal Chem 382:947–956CrossRefGoogle Scholar
  10. 10.
    Moreno-Bondi MC, Navarro-Villoslada F, Ito-Pena E, Urraca JL (2008) Curr Anal Chem 4:316–340CrossRefGoogle Scholar
  11. 11.
    Lotierzo M, Henry OYF, Piletsky S, Tothill I, Cullen D, Kania M, Hock B, Turner APF (2004) Biosens Bioelectron 20:145–152CrossRefGoogle Scholar
  12. 12.
    Matsui J, Akamatsu K, Hara N, Miyoshi D, Nawafune H, Tamaki K, Sugimoto N (2005) Anal Chem 77:4282–4285CrossRefGoogle Scholar
  13. 13.
    Hänel C, Gauglitz G (2002) Anal Bioanal Chem 372:91–100CrossRefGoogle Scholar
  14. 14.
    Nopper D, Lammershop O, Wulff G, Gauglitz G (2003) Anal Bioanal Chem 377:608–613CrossRefGoogle Scholar
  15. 15.
    Belmont AS, Jaeger S, Knopp D, Niessner R, Gauglitz G, Haupt K (2007) Biosens Bioelectron 22:3267–3272CrossRefGoogle Scholar
  16. 16.
    O’Shannessy DJ, Br E, Mosbach K (1989) Anal Biochem 177:144–149CrossRefGoogle Scholar
  17. 17.
    Weber A, Dettling M, Brunner H, Tover GEM (2002) Macromol Rapid Comm 23:824–828CrossRefGoogle Scholar
  18. 18.
    Mohrle BP, Kumpf M, Gauglitz G (2005) Analyst 130:1634–1638CrossRefGoogle Scholar
  19. 19.
    Lehmann M, Dettling M, Brunner H, Tovar GEM (2004) J Chrom B 808:43–50CrossRefGoogle Scholar
  20. 20.
    Asenath Smith E, Chen W (2008) Langmuir 24:12405–12409CrossRefGoogle Scholar
  21. 21.
    Mehne J, Markovic G, Pröll F, Schweizer N, Zorn S, Schreiber F, Gauglitz G (2008) Anal Bioanal Chem 391:1783–1791CrossRefGoogle Scholar
  22. 22.
    Tovar G, Kräuter I, Gruber C (2003) Top Curr Chem Springer Berlin/HeidelbergGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Felix Kolarov
    • 1
    Email author
  • Klaus Niedergall
    • 2
  • Monika Bach
    • 2
  • Günter E. M. Tovar
    • 2
  • Günter Gauglitz
    • 1
  1. 1.Institute of Physical and Theoretical ChemistryTübingen UniversityTübingenGermany
  2. 2.Institute for Interfacial Engineering IGVTUniversität StuttgartStuttgartGermany

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