Analytical and Bioanalytical Chemistry

, Volume 402, Issue 3, pp 1337–1346 | Cite as

Molecularly imprinted polymers for selective extraction of synephrine from Aurantii Fructus Immaturus

  • Jie-Ping FanEmail author
  • Lu Zhang
  • Xue-Hong Zhang
  • Jun-zhong Huang
  • Sheng Tong
  • Tao Kong
  • Zhe-You Tian
  • Jian-Hang Zhu
Original Paper


In this work, molecularly imprinted solid-phase extraction (MISPE) has been used to selectively enrich, purify, or remove synephrine from Aurantii Fructus Immaturus. To this end, a molecularly imprinted polymer (MIP) was prepared by self-assembly from the template synephrine, the functional monomer methacrylic acid, and the crosslinker ethylene glycol dimethacrylate in 1:4:20 molar ratio. Subsequent molecular interrogation of the MIP binding sites revealed preferential structural selectivity for synephrine relative to other structurally related naturally occurring compounds (i.e. octopamine and tyramine ). This selectivity was subsequently exploited to achieve substantial sample clean-up of extracts of crude Aurantii Fructus Immaturus and Aurantii Fructus Immaturus stir-baked with bran. The purity of synephrine in the extracts after MISPE represented approximately 24.21-fold enrichment of the synephrine in the untreated extracts of Aurantii Fructus Immaturus stir-baked with bran. High recoveries (85–90%) from the samples proved that the method was valid for selective enrichment, purification, or removal of synephrine from Aurantii Fructus Immaturus.


The Molecularly imprinted polymers for the selective extraction of synephrine


Molecularly imprinted polymers Solid-phase extraction Synephrine Aurantii Fructus Immaturus 



Financial support from the National Natural Science Foundation of China (nos 20806037 and 20876131), the Natural Science Foundation of Jiangxi, China (no. 2007GQH1752), the Natural Science Foundation of Jiangxi Province Department of Education (no. GJJ09420), the Open Foundation of Key Laboratory of Poyang Lake Ecology and Bio-Resource Utilization of Ministry of Education (no. Z04998), and Special Funds for Graduate Student Innovation in Jiangxi Province (no. YC09B012) are gratefully acknowledged. Chang-Xu Lin is thanked for operation of the SEM.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Jie-Ping Fan
    • 1
    • 2
    Email author
  • Lu Zhang
    • 2
  • Xue-Hong Zhang
    • 3
  • Jun-zhong Huang
    • 4
  • Sheng Tong
    • 2
  • Tao Kong
    • 2
  • Zhe-You Tian
    • 2
  • Jian-Hang Zhu
    • 1
    • 2
  1. 1.Key Laboratory of Poyang Lake Ecology and Bio-Resource Utilization of Ministry of EducationNanchang UniversityNanchangChina
  2. 2.School of Environmental and Chemical EngineeringNanchang UniversityNanchangChina
  3. 3.School of Foreign LanguageNanchang UniversityNanchangChina
  4. 4.Guang Dong Institute For Drug ControlGuangzhouChina

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