Surface plasmon resonance biosensor for the detection of VEGFR-1—a protein marker of myelodysplastic syndromes
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The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use.
KeywordsSurface plasmon resonance Myelodysplastic syndromes Vascular endothelial growth factor Protein markers
This study was supported by research grants NS10633-3/2009 and MZ 02373601 from the Ministry of Health, Czech Republic, by research grant KAN200670701 and Praemium Academiae from the Academy of Sciences, Czech Republic, and by Baxter, Czech Republic.
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