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Analytical and Bioanalytical Chemistry

, Volume 401, Issue 8, pp 2651–2656 | Cite as

Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips

  • Stefan Jezierski
  • Leonid Gitlin
  • Stefan Nagl
  • Detlev Belder
Technical Note

Abstract

We present a fast and versatile method to produce functional micro free-flow electrophoresis chips. Microfluidic structures were generated between two glass slides applying multistep liquid-phase lithography, omitting troublesome bonding steps or cost-intensive master structures. Utilizing a novel spacer-less approach with the photodefinable polymer polyethyleneglycol dimethacrylate (PEG-DA), microfluidic devices with hydrophilic channels of only 25 μm in height were generated. The microfluidic chips feature ion-permeable segregation walls between the electrode channels and the separation bed and hydrophilic surfaces. The performance of the chip is demonstrated by free-flow electrophoretic separation of fluorescent xanthene dyes and fluorescently labeled amino acids.

Keywords

Capillary electrophoresis/electrophoresis Microfluidics Microfabrication Free-flow electrophoresis Lab-on-a-chip 

Notes

Acknowledgments

S.J. is supported by the European Social Fund (ESF # 080938819) and S.N. by the Deutsche Forschungsgemeinschaft (DFG). The assistance of B. Kohlstrunk, Prof. J. A. Käs (mask aligner) and G. Ramm, Prof. M. Grundmann (surface profiles) from the Physics Department, University of Leipzig, is gratefully acknowledged.

Supplementary material

216_2011_5351_MOESM2_ESM.zip (5.9 mb)
Supplementary material, approximately 5.92 MB.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Stefan Jezierski
    • 1
  • Leonid Gitlin
    • 1
  • Stefan Nagl
    • 1
  • Detlev Belder
    • 1
  1. 1.Institut für Analytische ChemieUniversität LeipzigLeipzigGermany

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