Analytical and Bioanalytical Chemistry

, Volume 401, Issue 8, pp 2549–2559 | Cite as

Fast DNA and protein microarray tests for the diagnosis of hepatitis C virus infection on a single platform

  • Stuart W. J. Ember
  • Holger Schulze
  • Alan J. Ross
  • Julie Luby
  • Mizanur Khondoker
  • Gerard Giraud
  • Jonathan G. Terry
  • Ilenia Ciani
  • Chaker Tlili
  • Jason Crain
  • Anthony J. Walton
  • Andrew R. Mount
  • Peter Ghazal
  • Till T. Bachmann
  • Colin J. Campbell
Original Paper

Abstract

Hepatitis C virus (HCV) is a major cause of chronic liver disease and liver cancer, and remains a large health care burden to the world. In this study we developed a DNA microarray test to detect HCV RNA and a protein microarray to detect human anti-HCV antibodies on a single platform. A main focus of this study was to evaluate possibilities to reduce the assay time, as a short time-to-result (TTR) is a prerequisite for a point-of-care test. Significantly reducing hybridisation and washing times did not impair the assay performance. This was confirmed first using artificial targets and subsequently using clinical samples from an HCV seroconversion panel derived from a HCV-infected patient. We were able to reduce the time required for the detection of human anti-HCV antibodies to only 14 min, achieving nanomolar sensitivity. The protein microarray exhibited an analytical sensitivity comparable to that of commercial systems. Similar results were obtained with the DNA microarray using a universal probe which covered all different HCV genotypes. It was possible to reduce the assay time after PCR from 150 min to 16 min without any loss of sensitivity. Taken together, these results constitute a significant step forward in the design of rapid, microarray-based diagnostics for human infectious disease, and show that the protein microarray is currently the most favourable candidate to fill this role.

Keywords

DNA microarray Protein microarray HCV Time-to-result Point-of-care Seroconversion panel 

Supplementary material

216_2011_5332_MOESM1_ESM.pdf (301 kb)
Supplementary material, approximately 308 KB.
216_2011_5332_MOESM2_ESM.zip (1.4 mb)
Supplementary material, approximately 1.37 MB.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Stuart W. J. Ember
    • 1
  • Holger Schulze
    • 1
  • Alan J. Ross
    • 1
  • Julie Luby
    • 1
  • Mizanur Khondoker
    • 1
  • Gerard Giraud
    • 2
  • Jonathan G. Terry
    • 4
  • Ilenia Ciani
    • 5
  • Chaker Tlili
    • 5
  • Jason Crain
    • 2
    • 3
  • Anthony J. Walton
    • 4
  • Andrew R. Mount
    • 5
  • Peter Ghazal
    • 1
  • Till T. Bachmann
    • 1
  • Colin J. Campbell
    • 1
    • 5
  1. 1.Division of Pathway Medicine, College of Medicine and Veterinary MedicineThe University of EdinburghEdinburghUK
  2. 2.School of Physics and AstronomyThe University of EdinburghEdinburghUK
  3. 3.National Physical LaboratoryTeddingtonUK
  4. 4.Institute of Integrated Systems, School of EngineeringThe University of EdinburghEdinburghUK
  5. 5.EaStCHEM School of ChemistryThe University of EdinburghEdinburghUK

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