Analytical and Bioanalytical Chemistry

, Volume 402, Issue 8, pp 2625–2631 | Cite as

Fluorescence dye as novel label molecule for quantitative SERS investigations of an antibiotic

  • Anne März
  • Sabine Trupp
  • Petra Rösch
  • Gerhard J. Mohr
  • Jürgen Popp
Original Paper


Within this contribution, the proof-of-principle for a new concept for indirect surface-enhanced Raman spectroscopy (SERS) detection is presented. The fluorescence dye FR-530 is applied as a label molecule for the antibiotic erythromycin. The antibiotic binds directly to the label molecule. Changes within the SERS spectrum of the fluorescence dye appearing with the presence of the antibiotic are utilized for the detection and quantitative investigations of erythromycin. With the new concept of binding the label molecule directly to the analyte molecule, the application of linkage compounds like antibodies or any other recognition molecules becomes dispensable.


SERS Label molecules Antibiotic Microfluidic 



We gratefully acknowledge the Free State of Thuringia and the European Union (EFRE) for financial support under support code 2008FE9112 (BioOptiSens). We thank the microfluidic group of the IPHT for providing the lab-on-a-chip devices for the measurements. S. Trupp and G. J. Mohr were kindly supported by the Bayerische Staatsministerium für Wirtschaft, Infrastruktur, Verkehr und Technologie within project AZ-Nr.: 20.10-3410-2 (Projekt Sensormaterialien).

Supplementary material

216_2011_5273_MOESM1_ESM.pdf (258 kb)
Supplementary material Fig. S1–S3 (PDF 258 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Anne März
    • 1
  • Sabine Trupp
    • 2
  • Petra Rösch
    • 1
  • Gerhard J. Mohr
    • 2
  • Jürgen Popp
    • 1
    • 3
  1. 1.Institute of Physical Chemistry and Abbe Center of PhotonicsFriedrich-Schiller University JenaJenaGermany
  2. 2.Fraunhofer Research Institution for Modular Solid-State Technologies EMFTWorkgroup Sensor MaterialsRegensburgGermany
  3. 3.Institute of Photonic Technology (IPHT)JenaGermany

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