Analytical and Bioanalytical Chemistry

, Volume 402, Issue 8, pp 2485–2498 | Cite as

Direct analysis of dried blood spots coupled with mass spectrometry: concepts and biomedical applications

  • Julien Déglon
  • Aurélien Thomas
  • Patrice Mangin
  • Christian Staub


Because of the emergence of dried blood spots (DBS) as an attractive alternative to conventional venous plasma sampling in many pharmaceutical companies and clinical laboratories, different analytical approaches have been developed to enable automated handling of DBS samples without any pretreatment. Associated with selective and sensitive MS–MS detection, these procedures give good results in the rapid identification and quantification of drugs (generally less than 3 min total run time), which is desirable because of the high throughput requirements of analytical laboratories. The objective of this review is to describe the analytical concepts of current direct DBS techniques and to present their advantages and disadvantages, with particular focus on automation capacity and commercial availability. Finally, an overview of the different biomedical applications in which these concepts could be of major interest will be presented.


Direct analysis of dried blood spots


Dried blood spots Direct LC–MS–MS analysis Ambient MS Automated on-line DBS 


Conflict of interest/disclosure

The authors declare no conflicts of interest regarding this manuscript.

Supplementary material

216_2011_5161_MOESM1_ESM.pdf (95 kb)
Supplementary Material(PDF 95 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Julien Déglon
    • 1
    • 2
  • Aurélien Thomas
    • 1
    • 2
  • Patrice Mangin
    • 1
  • Christian Staub
    • 1
    • 2
  1. 1.Unit of Toxicology, CURMLGeneva 4Switzerland
  2. 2.Swiss Centre for Applied Human ToxicologyUniversity of GenevaGenevaSwitzerland

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