Detecting small lung tumors in mouse models by refractive-index microradiology
Refractive-index (phase-contrast) radiology was able to detect lung tumors less than 1 mm in live mice. Significant micromorphology differences were observed in the microradiographs between normal, inflamed, and lung cancer tissues. This was made possible by the high phase contrast and by the fast image taking that reduces the motion blur. The detection of cancer and inflammation areas by phase contrast microradiology and microtomography was validated by bioluminescence and histopathological analysis. The smallest tumor detected is less than 1 mm3 with accuracy better than 1 × 10−3 mm3. This level of performance is currently suitable for animal studies, while further developments are required for clinical application.
KeywordsLung cancer Synchrotron X-ray imaging Real-time imaging
We thank Ms. Yijing Guan for computer processing and Dr. Cyril Petibois for fruitful discussion. This work was supported by NNSF of 11079049 (China) and by CAS of KJCX3,SYW.N3 (China), NPST for Nanoscience and Nanotechnology, Thematic Program of Academia Sinica, the Biomedical NanoImaging Core Facility(Taiwan), Fonds National Suisse, Lausanne Center for Biomedical Imaging (CIBM), and the Creative Research Initiatives (Functional X-ray Imaging) of MOST/KOSEF (Korea).
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